Phospho-c-Jun-S73 Rabbit pAb - P05412

Core Product Specifications and Parameters

Biological Background: c-Jun Function and Localization

• c-Jun is a transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3' to regulate gene expression. Related references: PMID:10995748, PMID:22083952
• It heterodimerizes with FOS family proteins to form the AP-1 transcription complex, enhancing DNA binding and transcriptional activity.
• c-Jun, together with FOSB, mediates activation-induced T-cell death by binding to the AP-1 site of the FASLG/CD95L promoter and inducing its transcription upon TCR/CD3 signaling. Related references: PMID:12618758
• Phosphorylation at Serine 73 by HIPK3 promotes NR5A1 activity, increasing steroidogenic gene expression via cAMP signaling. Related references: PMID:17210646
• In colorectal cancer, c-Jun is involved in KRAS-mediated transcriptional activation of USP28, binding directly to the USP28 promoter. Related references: PMID:24623306
• c-Jun localizes to the nucleus and is expressed in developing and adult prostate tissues and prostate cancer cells.
• Post-translational modifications include phosphorylation, acetylation, and ubiquitination, which regulate its activity and stability.
• Upon Epstein-Barr virus infection, c-Jun activates viral BZLF1 expression by binding to the Z promoter.

Experimental Guidance and Technical Tips

• For WB, preserve phosphorylation by including phosphatase inhibitors in lysis and running buffers; consider treating samples with stimuli known to induce c-Jun phosphorylation at S73 (e.g., serum, growth factors, or stress).
• Validate antibody specificity using non-phosphorylated peptide blocking or phosphatase treatment to confirm phospho-specific signal.
• For IHC-P, optimize antigen retrieval and include phosphatase inhibitors during processing; evaluate phospho-signal in tissue sections known to express activated c-Jun.
• Due to cross-reactivity with mouse and rat, validate performance in the relevant species sample system, as antigen sequences may vary.

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CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials for transcription and oncology research, including this Phospho-c-Jun-S73 Rabbit pAb. We supply validated antibody pairs, optimized polyclonal antibodies, bulk ancillary reagents, and can source rare targets to streamline your diagnostic development from concept to clinic.