Phospho-c-Jun-S63 Rabbit pAb - P05412

Core Product Specifications and Parameters

Biological Background: Transcription factor Jun Function and Localization

• Recognizes and binds to the AP-1 consensus DNA motif 5'-TGA[GC]TCA-3' to regulate gene expression. Related references: PMID:10995748 PMID:22083952
• Heterodimerizes with FOS family proteins to form the AP-1 transcription complex, enhancing DNA binding and transcriptional activity.
• Together with FOSB, mediates activation-induced cell death of T cells by binding to the AP-1 site in the FASLG/CD95L promoter and inducing its transcription in response to TCR/CD3 signaling. Related references: PMID:12618758
• When phosphorylated by HIPK3, promotes NR5A1 activity and upregulates steroidogenic gene expression via the cAMP signaling pathway. Related references: PMID:17210646
• Participates in KRAS-mediated transcriptional activation of USP28 in colorectal cancer (CRC) cells by binding to the USP28 promoter. Related references: PMID:24623306
• Upon Epstein-Barr virus (EBV) infection, binds to the viral BZLF1 Z promoter and activates BZLF1 expression.
• Subcellularly localized to the nucleus and expressed in the developing and adult prostate as well as prostate cancer cells.
• c-Jun is a phosphoprotein with key regulatory phosphorylation at Ser63; it also undergoes acetylation and ubiquitination-like modifications.

Experimental Guidance and Technical Tips

• For western blotting, include phosphatase inhibitors during sample preparation to preserve the phospho-S63 epitope; the expected band for c-Jun is ~36 kDa.
• In IHC-P, consider using dephosphorylation controls to confirm phospho-specific nuclear staining.
• The synthetic phosphopeptide immunogen ensures specificity for the phosphorylated Ser63 residue; validate in relevant cellular models.
• For ELISA applications, the antibody can be used to detect phosphorylated c-Jun peptides or proteins.

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