Phospho-c-Jun-S63 Rabbit pAb for WB and ELISA - P05412

Core Product Specifications and Parameters

Biological Background: Transcription Factor Jun Function and Localization

• Transcription factor that recognizes and binds to the AP-1 consensus motif 5'-TGA[GC]TCA-3', regulating gene expression. Related references: PMID:10995748, PMID:22083952
• Heterodimerizes with FOS family proteins to form the AP-1 complex, enhancing DNA binding and transcriptional activity.
• Plays a role in activation-induced T cell death by binding to the FASLG/CD95L promoter and inducing its transcription upon TCR/CD3 signaling. Related references: PMID:12618758
• Phosphorylated by HIPK3, promotes NR5A1 activity to increase steroidogenic gene expression via the cAMP pathway. Related references: PMID:17210646
• Involved in KRAS-mediated transcriptional activation of USP28 in colorectal cancer cells, binding to the USP28 promoter. Related references: PMID:24623306
• Upon EBV infection, binds to viral BZLF1 Z promoter and activates BZLF1 expression.
• Located in the nucleus, consistent with its role as a transcription factor.
• Expressed in developing and adult prostate and prostate cancer cells, suggesting a role in prostate biology.

Experimental Guidance and Technical Tips

• The immunogen is a phosphopeptide around S63, so the antibody likely recognizes the phosphorylated form specifically. Consider using phosphatase-treated lysates as a negative control in Western blot to confirm phospho-specificity.
• Validated for WB and ELISA. For WB, a band at ~36 kDa is expected; optimal dilution should be determined empirically. For ELISA, coating with phospho-peptide and non-phospho-peptide may help assess specificity.
• Cross-reacts with human and rat; validate reactivity in the appropriate tissue or cell line expressing c-Jun.
• Because c-Jun phosphorylation is dynamic, include appropriate stimulation/inhibition conditions in experiments to modulate S63 phosphorylation (e.g., growth factors, stress signals).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers comprehensive access to IVD raw materials, supporting researchers and diagnostic developers studying c-Jun/AP-1 signaling and transcription. From validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and rare‑target sourcing, we bridge your project from concept to clinic with reliable supply solutions.