Phospho-c-Abl-Y412 Rabbit Polyclonal Antibody for WB, IHC-P, ELISA - P00519

Core Product Specifications and Parameters

Biological Background: Tyrosine-protein kinase ABL1 Function and Localization

• Non-receptor tyrosine-protein kinase ABL1, also known as c-Abl, p150, and Abelson tyrosine-protein kinase 1, is encoded by the ABL1 gene. It functions as a key regulator of cytoskeleton dynamics, cell adhesion, motility, endocytosis, autophagy, DNA damage response, and apoptosis.
• ABL1 phosphorylates a wide array of substrates to coordinate actin remodeling. This includes proteins like WASF3 (lamellipodia formation), ANXA1, DBN1, DBNL, CTTN, RAPH1, ENAH, MAPT, and PXN.
• Regulates cell adhesion and motility through phosphorylation of BCAR1, CRK, CRKL, DOK1, EFS, and NEDD9.
  Related references:
  PMID:22810897
• In response to oxidative stress, ABL1 translocates to mitochondria, leading to mitochondrial dysfunction and cell death. It phosphorylates PRKD2 at Tyr-717.
  Related references:
  PMID:28428613
• Translocates to the nucleus where it participates in DNA damage response and apoptosis. It phosphorylates DNA repair proteins (DDB1, DDB2, ERCC3, ERCC6, RAD9A, RAD51, RAD52, WRN) and proapoptotic factor TP73.
• The protein is localized in the cytoplasm, cytoskeleton, nucleus, mitochondrion, and nucleus membrane. It is widely expressed across tissues.
• ABL1 is a proto-oncogene. Chromosomal rearrangement with BCR generates the BCR-ABL fusion protein, a hallmark of chronic myeloid leukemia, leading to constitutive kinase activity.
• Keywords: Acetylation, alternative splicing, apoptosis, ATP-binding, autophagy, cell adhesion, chromosomal rearrangement, cytoplasm, cytoskeleton, disease variant, DNA damage, DNA repair, DNA-binding, endocytosis, kinase, lipoprotein, magnesium, manganese, membrane, metal-binding, mitochondrion, myristate, nucleotide-binding, nucleus, phosphoprotein, proteomics identification, proto-oncogene, reference proteome, SH2 domain, SH3 domain, transferase, tyrosine-protein kinase, Ubl conjugation.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphopeptide corresponding to the region around Y412 of human ABL1. It is predicted to specifically detect phosphorylation at tyrosine 412 in human, mouse, and rat samples.
• For Western blot (WB), immunohistochemistry (IHC-P), and ELISA applications, optimal antibody dilutions and incubation conditions should be determined empirically using positive and negative controls.
• To preserve phosphorylation signals, it is recommended to include phosphatase inhibitors in lysis buffers and to process samples quickly.
• Validate detection in cell lines or tissue samples known to express phospho-c-Abl, such as those stimulated with growth factors or subjected to oxidative stress.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with a comprehensive one-stop sourcing solution for IVD raw materials. This phospho-c-Abl (Y412) rabbit polyclonal antibody supports investigations into kinase signaling, oncogenesis, and cellular stress responses. Whether you need validated antibody pairs, optimized monoclonals or polyclonals, bulk ancillary reagents, or custom sourcing for rare targets, CamelBio simplifies your procurement from concept to clinic.