Phospho-c-Abl-Y412 Rabbit pAb - P00519

Core Product Specifications and Parameters

Biological Background: c-Abl Function and Localization

• c-Abl, encoded by ABL1, is a non-receptor tyrosine-protein kinase proto-oncogene also known as Abelson tyrosine-protein kinase 1, p150, and BCR-ABL (in leukemogenic fusions).
• It coordinates actin cytoskeleton remodeling, cell motility, adhesion, and endocytosis by phosphorylating effectors such as WASF3, DBNL, CTTN, and ENAH (PubMed:22810897). Related references: PMID:22810897
• In response to oxidative stress, ABL1 translocates to mitochondria, phosphorylates PRKD2 on Tyr‑717, and promotes mitochondrial dysfunction and apoptosis (PubMed:28428613). Related references: PMID:28428613
• Nuclear ABL1 participates in DNA‑damage sensing and repair, interacting with factors such as DDB1, RAD51, and WRN; it also triggers TP73‑dependent apoptosis when damage is overwhelming.
• A critical regulatory autophosphorylation site is Tyr‑412 (the target of this antibody), which lies in the activation loop and is essential for kinase activity.
• ABL1 is widely expressed and localizes to the cytoplasm, cytoskeleton, nucleus, and mitochondria, reflecting its diverse roles.
• It carries numerous post‑translational modifications (phosphorylation, acetylation, myristoylation, ubiquitination) and interacts with SH2/SH3‑domain‑containing adaptor proteins.
• Pathogens such as Helicobacter pylori (CagA) and Vaccinia virus hijack ABL1 signaling to reorganize the host actin cytoskeleton for infection.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phosphopeptide corresponding to Tyr‑412 of human ABL1; we recommend validating specificity by comparing signals in untreated vs. phosphatase‑treated lysates.
• For IHC‑P, consider standard antigen retrieval methods (e.g., citrate buffer, pH 6.0) and test a range of primary antibody dilutions to optimize phospho‑signal detection.
• In ELISA, use phosphorylated and non‑phosphorylated peptide controls to confirm phospho‑specificity and cross‑reactivity.
• The reported cross‑reactivity with mouse and rat suggests conserved epitope sequences; however, always verify reactivity in your specific model system.

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