Phospho-BRD4-T249 Rabbit pAb for WB - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• BRD4 (Bromodomain-containing protein 4, also known as HUNK1) is a chromatin reader that recognizes and binds acetylated histones, playing a key role in the transmission of epigenetic memory across cell divisions and transcription regulation. Related references: PMID:20871596 PMID:23086925 PMID:23317504
• It remains associated with acetylated chromatin throughout the entire cell cycle, preserving acetylated chromatin status and maintaining higher-order chromatin structure for postmitotic G1 gene transcription. Related references: PMID:22334664 PMID:23317504 PMID:23589332
• During interphase, BRD4 regulates the transcription of signal-inducible genes by recruiting the P-TEFb complex to promoters and distal enhancers, facilitating RNA polymerase II CTD phosphorylation and productive elongation. Related references: PMID:16109376 PMID:16109377 PMID:19596240
• BRD4 binds acetylated RELA to activate NF-kappa-B signaling, and following CK2 phosphorylation, is recruited to p53/TP53 target promoters to regulate p53-mediated transcription. Related references: PMID:19103749 PMID:23317504
• In the DNA damage response, BRD4 acts as a chromatin insulator by recruiting the condensin-2 complex to acetylated histones, limiting spreading of histone H2AX phosphorylation.
• Ubiquitously expressed and localizes to the nucleus and chromatin.
• Post-translationally modified by acetylation and phosphorylation; contains two bromodomains; involved in chromosomal rearrangements linked to cancer; interacts with viral proteins.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr249 of human BRD4. To confirm phosphorylation specificity, consider using a non-phospho-specific BRD4 antibody as a control in parallel experiments.
• For western blotting (WB), a band of approximately 152 kDa is expected. Use a suitable gel percentage (e.g., 8% polyacrylamide) and optimize transfer conditions for high-molecular-weight proteins.
• Validate the antibody in a relevant cell line (e.g., HeLa, Jurkat) with known BRD4 phosphorylation status. Include phosphatase inhibitor cocktails in lysis buffers to preserve phosphorylation signals.
• Since cross-reactivity is reported for mouse and rat, this antibody may be suitable for rodent model studies; include appropriate species-specific positive controls for verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a trusted one-stop partner for diagnostic manufacturers, research institutes, and laboratories, providing a comprehensive range of IVD raw materials and technical support. For epigenetic research targets such as BRD4, we offer validated antibody reagents, including phosphorylated-specific clones, matched antibody pairs, and bulk ancillary supplies. Our robust sourcing capabilities help streamline your workflow from concept to clinic, ensuring reliable access to high-quality critical raw materials.