Phospho-BRD4-T204 Rabbit pAb - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• Bromodomain-containing protein 4 (BRD4), also known as HUNK1, is a chromatin reader that recognizes and binds acetylated histones, playing a key role in epigenetic memory and transcription regulation. Related references: PMID:20871596, PMID:23086925, PMID:23317504
• It remains associated with acetylated chromatin throughout the cell cycle, preserving chromatin status for postmitotic G1 gene transcription. Related references: PMID:22334664, PMID:23317504, PMID:23589332
• BRD4 regulates signal-inducible gene transcription by recruiting the P-TEFb complex to promoters and distal enhancers, facilitating phosphorylation of RNA polymerase II's C-terminal domain. Related references: PMID:16109376, PMID:16109377, PMID:19596240
• Beyond histones, it binds acetylated RELA, activating NF-kappa-B signaling. Related references: PMID:19103749
• Phosphorylation by CK2 recruits BRD4 to p53/TP53-specific target promoters, modulating p53-mediated transcription. Related references: PMID:23317504
• It acts as a chromatin insulator in DNA damage response by recruiting condensin-2 to acetylated histones, limiting H2AX phosphorylation spreading.
• Subcellular localization is primarily nuclear, associated with chromosomes.
• Ubiquitously expressed across tissues, with implications in various regulatory processes.

Experimental Guidance and Technical Tips

• Since this antibody targets phosphorylation at T204, ensure that samples are prepared with phosphatase inhibitors to maintain phospho-signal.
• For Western blot, consider using appropriate loading controls and validate band specificity by phosphatase treatment or mutant controls.
• ELISA may require optimization of coating antigen and detection conditions.
• Cross-reactivity with mouse and rat suggests a conserved epitope; verify performance in the relevant species' sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as your comprehensive IVD raw-material partner, providing validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents for targets like BRD4 in epigenetic and transcription research. We assist diagnostic manufacturers and laboratories in securing rare-target raw materials efficiently. From concept to clinic, rely on CamelBio for streamlined sourcing and technical support.