Phospho-BRD4 (S492/S494) Rabbit Polyclonal Antibody for WB, DB, ELISA - O60885

Core Product Specifications and Parameters

Biological Background: BRD4 Function and Localization

• BRD4 is a member of the bromodomain and extraterminal (BET) family, functioning as a chromatin reader that binds acetylated histones to transmit epigenetic memory across cell divisions. Related references: PMID:20871596, PMID:23086925, PMID:23317504
• It remains associated with acetylated chromatin throughout the cell cycle, maintaining high-order chromatin structure for postmitotic G1 gene transcription. Related references: PMID:22334664, PMID:23317504, PMID:23589332
• BRD4 recruits the P-TEFb complex to gene promoters and distal enhancers, leading to RNA polymerase II CTD phosphorylation and activation of signal-inducible transcription. Related references: PMID:16109376, PMID:16109377, PMID:19596240
• It also recruits P-TEFb to acetylated RELA, linking BRD4 to NF-kappa-B pathway activation. Related references: PMID:19103749
• Phosphorylation at residues such as S492 and S494 by CK2 facilitates BRD4's interaction with p53/TP53-targeted promoters. Related references: PMID:23317504
• In the DNA damage response, BRD4 acts as a chromatin insulator by recruiting condensin-2 to acetylated histones, limiting H2A.X phosphorylation spreading.
• BRD4 localizes to the nucleus and chromosomes; it is ubiquitously expressed and undergoes alternative splicing and post-translational modifications including phosphorylation, acetylation, and ubiquitination.
• Additionally, BRD4 regulates CD4+ T-cell differentiation into Th17 cells (By similarity).

Experimental Guidance and Technical Tips

• For Western blot, prepare lysates with phosphatase inhibitors to preserve phosphorylation and use a low-percentage (e.g., 6–8%) SDS-PAGE gel for optimal separation of the ~152 kDa BRD4 protein.
• Dot blot and ELISA assays can benefit from blocking with a phospho-free BSA to reduce background; confirm specificity using non-phosphorylated peptide competition.
• The antibody’s reactivity in human samples suggests its use with human cell lines; validate cross-reactivity in your target system as needed.
• Consider using appropriate positive controls, such as lysates from cells treated with phosphatase inhibitors or known phosphorylation inducers.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio bridges the gap from concept to clinic by supplying IVD manufacturers and researchers with high-quality raw materials. This Phospho-BRD4 (S492/S494) rabbit polyclonal antibody is part of our portfolio for chromatin and transcription studies, backed by our comprehensive sourcing and technical support services.