Phospho-BRCA1-S1423 Rabbit pAb - P38398

Core Product Specifications and Parameters

Biological Background: BRCA1 Function and Localization

• BRCA1 functions as an E3 ubiquitin-protein ligase that specifically mediates the formation of 'Lys-6'-linked polyubiquitin chains and plays a central role in DNA repair by facilitating cellular responses to DNA damage. Related references: PMID:10500182 PMID:12887909 PMID:12890688

• The BRCA1-BARD1 heterodimer coordinates diverse cellular pathways including DNA damage repair, ubiquitination, and transcriptional regulation to maintain genomic stability. Related references: PMID:12890688 PMID:14976165 PMID:20351172

• BRCA1 is required for appropriate cell cycle arrests after ionizing irradiation in both the S-phase and the G2 phase of the cell cycle. Related references: PMID:10724175 PMID:11836499 PMID:12183412

• Contributes to homologous recombination repair (HRR) via direct interaction with PALB2, modulating the loading of BRCA2-RAD51 repair machinery at DNA breaks. Related references: PMID:19369211

• Component of the BRCA1-RBBP8 complex that regulates CHEK1 activation and controls cell cycle G2/M checkpoints upon DNA damage through BRCA1-mediated ubiquitination of RBBP8. Related references: PMID:16818604

• Subcellular localization includes the nucleus, chromosomes, and cytoplasm. Isoform 1 and isoform 3 are widely expressed, with isoform 3 often reduced or absent in breast and ovarian cancer cell lines.

Experimental Guidance and Technical Tips

• This phospho-specific antibody is suitable for Western blotting (WB), immunohistochemistry (IHC-P), immunofluorescence (IF/ICC), and ELISA applications. For WB, expect a band at approximately 208 kDa; include phosphatase-treated controls or peptide competition to confirm phosphorylation specificity.
• Cross-reactivity with human, mouse, and rat samples is indicated, but optimal working dilutions should be determined empirically for each assay and species.
• For IHC and IF, antigen retrieval and blocking conditions may need optimization depending on tissue type and fixation methods.
• Consider nuclear and cytoplasmic localization patterns consistent with BRCA1 biology when interpreting IF or IHC results.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a reliable one-stop partner for IVD raw material sourcing, supporting research from concept to clinic. This anti-phospho-BRCA1 (S1423) rabbit polyclonal antibody is tailored for DNA damage and oncology research. Our comprehensive portfolio includes validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and custom sourcing for rare targets, ensuring seamless integration into your diagnostic or research workflows.