Phospho-Beclin 1-Ser93 Rabbit pAb for WB, ELISA - Q14457

Core Product Specifications and Parameters

Biological Background: Beclin-1 Function and Localization

• Beclin-1, encoded by the BECN1 gene, is also known as Coiled-coil myosin-like BCL2-interacting protein and Protein GT197.
• Central autophagy regulator: acts as a core subunit of the PI3K complex that produces phosphatidylinositol 3-phosphate; PI3KC3-C1 drives autophagosome initiation, while PI3KC3-C2 handles autophagosome maturation and endocytosis. Related references: PMID:18570871, PMID:21358617, PMID:23184933
• Regulates degradative endocytic trafficking and is required for the abscission step in cytokinesis, likely through the PI3KC3-C2 complex. Related references: PMID:20208530, PMID:20643123, PMID:23974797
• Involved in endocytosis. Related references: PMID:25275521
• A 35 kDa mitochondrial fragment (Beclin-1-C) promotes apoptosis by inducing BAX translocation and the release of proapoptotic factors.
• Protects against infection by a neurovirulent strain of Sindbis virus.
• Localizes to multiple compartments: cytoplasm, Golgi apparatus, endosomes, endoplasmic reticulum, mitochondria, autophagosomes, and nucleus.
• Ubiquitously expressed across tissues.
• Undergoes multiple post‑translational modifications including phosphorylation and acetylation; annotated under processes such as autophagy, apoptosis, antiviral defense, cell cycle, and host‑virus interactions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide corresponding to Ser93; use phosphatase inhibitors during sample preparation to preserve the phosphorylation signal.
• In WB, the full‑length Beclin-1 band is expected at approximately 52 kDa. Cleavage fragments (e.g., the ~35 kDa mitochondrial form) may also be detected.
• The antibody is validated for WB and ELISA; performance in other applications should be experimentally optimized.
• Cross‑reactivity is confirmed for human; for other species, validate specificity in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your dedicated partner in sourcing high‑quality IVD raw materials, offering validated antibody pairs, recombinant antibodies, and bulk ancillary reagents. This Phospho-Beclin 1‑Ser93 antibody supports research in autophagy, apoptosis, and infectious disease, delivering reliable performance for diagnostic development. From rare‑target raw materials to comprehensive technical consulting, CamelBio bridges the gap from concept to clinical application.