Phospho-Beclin 1 (Ser90) Rabbit Polyclonal Antibody for WB and ELISA - Q14457

Core Product Specifications and Parameters

Biological Background: Beclin-1 Function and Localization

• Beclin-1 (ATG6/VPS30) is a core component of the class III phosphatidylinositol 3-kinase (PI3KC3) complexes, essential for autophagy and membrane trafficking. Related references: PMID:18570871 PMID:21358617 PMID:23184933
• It regulates distinct steps of autophagy: the PI3KC3-C1 complex initiates autophagosome formation, while PI3KC3-C2 promotes autophagosome maturation and endocytosis. Related references: PMID:20208530 PMID:20643123 PMID:23974797
• Beclin-1 participates in endocytic trafficking and is required for the abscission step of cytokinesis. Related references: PMID:25275521
• A C-terminal 35 kDa fragment of Beclin-1 localizes to mitochondria, promoting apoptosis by facilitating BAX translocation and cytochrome c release.
• Subcellularly, Beclin-1 is found in the cytoplasm, Golgi apparatus, trans-Golgi network, endosomes, endoplasmic reticulum, mitochondria, autophagosomes, and nucleus, reflecting its multifunctional roles.
• Beclin-1 exhibits broad tissue expression (ubiquitous) and is regulated by various post-translational modifications, including phosphorylation (e.g., at Ser90) and acetylation.
• It plays a role in antiviral host defense and protects against neurotropic Sindbis virus infection.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phospho-peptide corresponding to Ser90 of human Beclin-1; therefore, the antibody is expected to specifically recognize the phosphorylated form. Include phosphatase inhibitors in lysis buffers to preserve phosphorylation.
• For Western blot, the predicted molecular weight of full-length Beclin-1 is ~52 kDa. A cleaved ~35 kDa fragment may be detected under apoptotic conditions.
• For ELISA, phosphorylation-specific detection may be confirmed by peptide competition assay using non-phosphorylated and phosphorylated peptides.
• Cross-reactivity with mouse and rat indicates epitope conservation; however, validation in the specific experimental system is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop sourcing partner for IVD raw materials, offering rapid access to validated antibody pairs, bulk ancillary reagents, and customized monoclonal/polyclonal antibodies. This phospho-Beclin-1 (Ser90) antibody supports autophagy and apoptosis research, key areas for diagnostic and therapeutic development. Leverage our expertise in rare-target raw-material sourcing to streamline your assay development pipeline.