Phospho-Beclin 1 (Ser15) Rabbit Polyclonal Antibody for WB and ELISA - Q14457

Core Product Specifications and Parameters

Biological Background: Beclin-1 Function and Localization

• Beclin-1 (also known as coiled-coil myosin-like BCL2-interacting protein, ATG6, or VPS30) is a core autophagy protein encoded by the BECN1 gene.
• Beclin-1 is a central autophagy protein that functions as a core subunit of the class III PI3K complex, mediating production of phosphatidylinositol 3-phosphate. Related references: PMID:18570871 PMID:21358617 PMID:23184933
• It is essential for the formation of distinct PI3K complexes: PI3KC3-C1 for autophagosome initiation and PI3KC3-C2 for autophagosome maturation and endocytosis. Related references: PMID:20208530 PMID:20643123 PMID:23974797
• Beclin-1 is involved in endocytic trafficking and the abscission step of cytokinesis. Related references: PMID:25275521
• A 35 kDa mitochondrial fragment of Beclin-1 promotes apoptosis by inducing BAX translocation and the release of proapoptotic factors.
• It localizes to multiple organelles: cytoplasm, Golgi, endosomes, endoplasmic reticulum, mitochondria, autophagosomes, and nucleus, consistent with its roles in membrane trafficking.
• Beclin-1 protects against infection by neurovirulent Sindbis virus, indicating a role in antiviral defense.
• Beclin-1 undergoes various post-translational modifications, including phosphorylation at Ser15, acetylation, and ubiquitination, which regulate its functions.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphopeptide specific to Ser15 of human Beclin-1. To confirm phospho-specificity, consider phosphatase-treated samples or peptide competition assays in your experimental setup.
• For Western blot, a band around 52 kDa is expected. Optimize using gradient gels and transfer conditions suitable for proteins of this molecular weight.
• For ELISA, direct coating of the phosphopeptide antigen may assist in assessing antibody binding; optimize coating buffers and blocking conditions for your plate format.
• Cross-reactivity with mouse and rat Beclin-1 is indicated; thus, cell lines or tissue lysates from these species can be used for functional autophagy studies.
• Phosphorylation of Beclin-1 can be transient; include phosphatase inhibitors in lysis buffers and process samples quickly to preserve the phospho-epitope.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials, technical services, and consulting, supporting every stage from concept to clinic. This anti-phospho-Beclin-1 (Ser15) antibody exemplifies our commitment to supplying specialized reagents for autophagy and apoptosis research. Beyond individual antibodies, we offer validated antibody pairs, bulk monoclonal and polyclonal antibodies, ancillary reagents, and custom sourcing for rare targets to streamline your assay development.