Phospho-Bcl-2-S87 Rabbit pAb for WB - P10415

Core Product Specifications and Parameters

Biological Background: Bcl-2 Function and Localization

• Bcl-2 suppresses apoptosis in various cell systems, including lymphohematopoietic and neural cells. Related references: PMID:1508712 PMID:8183370

• It regulates cell death by controlling mitochondrial membrane permeability, functioning in a feedback loop with caspases and inhibiting cytochrome c release. Related references: PMID:11368354

• Bcl-2 also acts as an inhibitor of autophagy by interacting with BECN1 and AMBRA1 under non-starvation conditions. Related references: PMID:18570871 PMID:20889974 PMID:21358617

• May attenuate inflammation by impairing NLRP1-inflammasome activation, thus reducing CASP1 activation and IL1B release. Related references: PMID:17418785

• Localizes to the mitochondrial outer membrane, as well as nuclear and endoplasmic reticulum membranes, and is found in the cytoplasm.

• The protein is subject to phosphorylation (e.g., Ser87 site recognized by this antibody), ubiquitination, and alternative splicing, with a molecular weight of ~26 kDa.

• Expressed in a variety of tissues; linked to chromosomal rearrangements in cancer, making it an important proto-oncogene and disease variant target.

Experimental Guidance and Technical Tips

• For WB, optimize antibody dilution and validate in relevant human or rat cell lines expressing endogenous Bcl-2.
• Since this antibody targets a phosphorylation-specific epitope, include phosphatase inhibitors during sample preparation to preserve the signal.
• For ELISA, confirm specificity by comparing phosphorylated versus non-phosphorylated peptide antigens.
• Expect a band at ~26 kDa; use appropriate molecular weight markers for verification.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports your Bcl-2 apoptosis and autophagy research with a comprehensive IVD raw-material portfolio—from this phospho-specific antibody to matched capture/detection pairs. Our supply network covers optimized monoclonals, recombinant antigens, and ancillary reagents, ensuring a reliable sourcing bridge for diagnostic developers. For specialized needs, our technical team can also assist with custom raw-material sourcing to accelerate your assay development.