Phospho-BCAR1 (Y410) Rabbit Polyclonal Antibody for WB, ELISA - P56945

Core Product Specifications and Parameters

Biological Background: BCAR1 Function and Localization

• BCAR1 (Breast cancer anti-estrogen resistance protein 1), also known as p130cas or CRK-associated substrate, is a docking protein encoded by the BCAR1 gene (GeneID: 9564).
• It plays a central coordinating role in tyrosine kinase-based signaling related to cell adhesion, implicated in cell migration and branching. Related references: PMID:12432078 PMID:12832404 PMID:17038317
• Involved in BCAR3-mediated inhibition of TGFB signaling (By similarity).
• Subcellular localization includes focal adhesions, cytoplasm, and cell projections such as axons.
• Tissue specificity: Expressed in B-cells, widely expressed with abundant testis expression, and low expression in liver, thymus, and peripheral blood leukocytes. Related references: PMID:9020138 PMID:10639512
• BCAR1 undergoes phosphorylation on multiple tyrosine residues, including Y410, which regulates its interaction with SH2 domain-containing proteins.
• Alternative splicing produces multiple isoforms; the canonical protein has a molecular weight of ~93 kDa.
• Belongs to the CAS family of scaffolding proteins, featuring an SH3 domain and SH3-binding motifs.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y410 of human BCAR1; confirm antibody specificity for the phosphorylated form using appropriate controls like non-phosphorylated peptide competition or phosphatase treatment.
• For Western blot (WB), the predicted molecular weight is 93 kDa; consider using a suitable gel percentage for optimal separation.
• This antibody has been validated for WB and ELISA with human and mouse samples; cross-reactivity with other species may require individual testing.
• The antibody is shipped on ice bags; upon arrival, store at appropriate conditions as recommended by the supplier to maintain activity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio supports diagnostic manufacturers and research institutes with a comprehensive portfolio of IVD raw materials. For BCAR1 and phosphorylation signaling research, we offer this high-quality rabbit polyclonal antibody alongside validated antibody pairs, bulk ancillary reagents, and custom sourcing services. Contact us to explore our full range of cell adhesion and cancer-related research tools tailored for assay development.