Phospho-BCAR1-Y165 Rabbit pAb for WB, ELISA - P56945

Core Product Specifications and Parameters

Biological Background: BCAR1 Function and Localization

• BCAR1 (p130cas) is a scaffolding protein belonging to the Cas family, critical for integrin-mediated signal transduction.
• BCAR1 acts as a central docking protein for tyrosine kinase-based signaling pathways triggered by integrin engagement, promoting cell adhesion, migration, and branching.
  Related references: PMID:12432078, PMID:12832404, PMID:17038317
• Alternatively spliced isoforms add functional diversity, potentially modulating scaffold interactions.
• Localizes predominantly to focal adhesions and the cytoplasm, and is also detected in neuronal axons.
• Tyrosine phosphorylation of BCAR1, including at Y165, facilitates recruitment of downstream effectors and is essential for functional signaling output.
• Tissue expression is broad but highest in testis; lower levels in liver, thymus, and leukocytes. At the protein level, it is detected in B-cells.
  Related references: PMID:9020138, PMID:10639512
• Involved in BCAR3-mediated inhibition of TGFB signaling, contributing to anti-estrogen resistance in breast cancer.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Y165; confirm phospho-specificity by comparing signals from phosphorylated and non-phosphorylated protein controls in your experimental system.
• For Western blotting, optimize blocking and antibody dilution to minimize background, especially when probing cell adhesion signaling phospho-targets.
• Validated for human, mouse, and rat reactivity; verify performance in relevant tissue or cell lysates before large-scale studies.

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CamelBio provides diagnostic manufacturers and researchers with streamlined access to high-quality IVD raw materials, including phospho-specific antibodies like this anti-BCAR1-Y165 product. Our portfolio encompasses validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents for cell adhesion and signaling research. Contact us to leverage our one-stop sourcing capabilities and accelerate your development pipeline from concept to clinic.