Phospho-Bad-S112 Rabbit mAb for WB, IHC-P, ELISA - Q92934

Core Product Specifications and Parameters

Biological Background: Bad (Bcl2-associated agonist of cell death) Function and Localization

• Bad (Bcl2-associated agonist of cell death, also known as Bcl-2-binding component 6, Bcl-2-like protein 8) is a pro-apoptotic member of the Bcl-2 family that promotes cell death by competing for binding to anti-apoptotic proteins Bcl-X(L), Bcl-2, and Bcl-W.
• This competition disrupts heterodimerization of Bcl-X(L) with BAX, thereby reversing the death-repressor activity of Bcl-X(L), though not of Bcl-2, and links growth factor receptor signaling to apoptosis (By similarity).
• The BAD gene (Gene ID: 572) encodes a single protein product with a molecular weight of approximately 18 kDa, as confirmed by UniProt (Q92934, 18392 Da).
• Bad is localized to the mitochondrial outer membrane and cytoplasm, where it integrates survival and death signals; its activity is tightly regulated by post-translational modifications.
• Multiple key post-translational modifications have been identified on Bad, including phosphorylation, acetylation, and methylation, which modulate its pro-apoptotic function and subcellular localization.
• Phosphorylation at Ser-112 (and surrounding sites) inactivates Bad by promoting binding to 14-3-3 proteins and sequestering it in the cytoplasm, preventing mitochondrial translocation and apoptosis.
• Bad is expressed in a wide variety of tissues, consistent with its fundamental role in regulating programmed cell death across different cell types.

Experimental Guidance and Technical Tips

• Because the immunogen is a synthetic phosphopeptide encompassing S112 of human Bad, this antibody is expected to specifically recognize the Ser-112-phosphorylated form; it is advisable to verify phospho-specificity using phosphatase-treated lysates or a non-phospho-specific Bad antibody as a control.
• For Western blot (WB) analysis, the predicted molecular weight of Bad is 18 kDa; a single band at approximately this size should be detected under optimal conditions, though users should validate the signal in the relevant sample system and experimental workflow.
• For immunohistochemistry (IHC-P), antigen retrieval and blocking conditions should be optimized empirically; the expected localization is mitochondrial and cytoplasmic, so staining patterns should be evaluated accordingly.
• In ELISA applications, this antibody can be used for capture or detection of phosphorylated Bad in cell or tissue lysates; it is recommended to validate the assay conditions with appropriate positive and negative controls.
• Given cross-reactivity with mouse and rat Bad, this antibody is suitable for studies in these common model organisms; however, users should confirm that the sequence around S112 is conserved in these species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a comprehensive supplier for IVD raw materials, offering validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare target sourcing to support diagnostic manufacturers and researchers. This phospho-specific Bad (S112) antibody exemplifies our commitment to providing high-quality reagents for apoptosis and cell death pathway research, enabling the development of robust immunoassays. For tailored procurement needs beyond catalog products, our sourcing experts can help locate custom or bulk raw materials to accelerate your diagnostic project from concept to clinic.