Phospho-B-Raf (S445) Polyclonal Antibody for WB, ELISA - P15056

Core Product Specifications and Parameters

Biological Background: B-Raf Function and Localization

• Serine/threonine-protein kinase that transduces mitogenic signals from the cell membrane to the nucleus by phosphorylating and activating MAP2K1, thereby triggering the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Related references: PMID:21441910, PMID:29433126
• Also phosphorylates PFKFB2, linking growth factor signaling to metabolic regulation (PubMed:36402789). Related references: PMID:36402789
• May play a role in postsynaptic responses of hippocampal neurons, suggesting involvement in neuronal signaling (PubMed:1508179). Related references: PMID:1508179
• Localizes to the nucleus, cytoplasm, and cell membrane, consistent with its role in signal relay.
• Primarily expressed in the brain and testis, with lower levels in other tissues.
• Undergoes extensive post-translational modifications including phosphorylation, acetylation, methylation, and ubiquitination, which regulate its activity and interactions.
• A proto-oncogene frequently mutated in human cancers; disease‑associated variants are linked to cardiomyopathy, deafness, ectodermal dysplasia, and intellectual disability (per UniProt keywords).

Experimental Guidance and Technical Tips

• This antibody was raised against a phosphopeptide corresponding to residues around S445 of human BRAF; it is expected to recognize B‑Raf only when phosphorylated at this site.
• For WB and ELISA, consider using phosphatase inhibitors during sample preparation to preserve the S445 phosphorylation status.
• When setting up WB, validate the signal by treating samples with lambda phosphatase or by comparing with non‑phospho‑specific B‑Raf antibodies.
• Optimal working dilutions for WB and ELISA should be determined empirically; use positive controls (e.g., stimulated cell lysates known to activate the MAPK pathway).
• Cross‑reactivity with rat B‑Raf has been confirmed; for other species, test experimentally as sequence alignment may suggest reactivity.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with comprehensive access to IVD raw materials, technical services, and consulting from concept to clinic. For your B‑Raf‑related projects—whether in oncology biomarker development, MAPK pathway research, or neurological studies—we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and expert support for rare‑target sourcing. Partner with CamelBio to streamline your raw‑material procurement and accelerate your assay development.