Phospho-ATF2-T71 Rabbit pAb for WB, IHC-P, ELISA - P15336

Core Product Specifications and Parameters

Biological Background: ATF-2 Function and Localization

• ATF-2 (Cyclic AMP-dependent transcription factor ATF-2) is a member of the ATF/CREB family of transcription factors; also known as CREB-2, HB16, CRE-BP1, and TREB7.
• It functions as a transcriptional activator, binding to CRE (5'-TGACGTCA-3') or AP-1 (5'-TGACTCA-3') consensus sequences depending on its binding partner, regulating genes involved in anti-apoptosis, cell growth, and DNA damage response.
• In the nucleus, ATF-2 participates in global transcription and DNA damage response. Upon phosphorylation by ATM, it contributes to ionizing radiation-induced S phase checkpoint control and recruitment of the MRN complex to DNA damage foci.
• ATF-2 also localizes to the cytoplasm and mitochondrial outer membrane, where it interacts with HK1 and VDAC1 to impair mitochondrial membrane potential, induce leakage, and promote cell death.
• It possesses intrinsic histone acetyltransferase (HAT) activity, specifically acetylating histones H2B and H4 in vitro. Related references: PMID:10821277
• Additionally, ATF-2 acts in a ubiquitin ligase complex with CUL3 and RBX1 to promote degradation of KAT5, thereby modulating ATM acetylation and activity.
• ATF-2 is ubiquitously expressed, with highest levels in the brain, and undergoes alternative splicing, acetylation, and phosphorylation (including at Thr71) as key regulatory modifications.
• Keywords associated with ATF-2 include DNA-binding, zinc-finger, transcription regulation, and tumor suppressor/oncogenic activities depending on tissue context.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic phosphorylated peptide surrounding Thr71 of human ATF2, ensuring specificity for the phosphorylated form. For detection of total ATF2 (unphosphorylated), consider using a pan-ATF2 antibody.
• In Western blot, expect a band at approximately 55 kDa (predicted molecular weight ~54.5 kDa). Validate phospho-specificity by treating samples with phosphatase or using a non-phosphorylated blocking peptide.
• For IHC-P, antigen retrieval conditions should be optimized for your tissue type; consider testing citrate or EDTA-based retrieval buffers.
• For ELISA, confirm optimal coating concentrations and detection range in your specific assay setup.
• The antibody cross-reacts with human, mouse, and rat. Sequence conservation around Thr71 in other species may allow broader reactivity; validate in the relevant sample system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with one-stop access to high-quality IVD raw materials, including phospho-specific antibodies like this ATF2 (Thr71) antibody for transcription and DNA damage response research. We support your workflow from concept to clinic with validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and sourcing for rare targets—ensuring you have the right tools at every stage.