Phospho-ATF2-T71 Rabbit mAb for WB and ELISA - P15336

Core Product Specifications and Parameters

Biological Background: ATF2 Function and Localization

• ATF2 is a cyclic AMP-dependent transcription factor that binds CRE (cAMP response element) and AP-1 consensus sequences, regulating genes involved in anti-apoptosis, cell growth, and DNA damage response.
• It localizes to the nucleus, cytoplasm, and mitochondrial outer membrane; its subcellular distribution influences its function in transcription and apoptosis.
• In the cytoplasm, ATF2 interacts with mitochondrial outer membrane complexes, impairing membrane potential and promoting cell death.
• It plays a role in the DNA damage response, particularly in ionizing radiation-induced S phase checkpoint control and MRN complex recruitment.
• ATF2 possesses intrinsic histone acetyltransferase (HAT) activity, acetylating histones H2B and H4 in vitro. Related references: PMID:10821277
• The protein is heavily regulated by phosphorylation; phosphorylation at Thr71 (T71) modulates its activity, and this antibody specifically recognizes the phosphorylated form.
• ATF2 is ubiquitously expressed, with higher levels in the brain, and alternative splicing generates multiple isoforms.
• It is a zinc-finger phosphoprotein involved in a variety of cellular processes, including oncogenesis and tumor suppression depending on the tissue context.

Experimental Guidance and Technical Tips

• As a phospho-specific antibody, experimental conditions that preserve protein phosphorylation (e.g., use of phosphatase inhibitors, gentle lysis) are critical.
• For Western blotting, optimize blocking and antibody dilution; consider including a phosphatase-treated control to confirm signal specificity.
• Validate the antibody’s reactivity with your target sample; for mouse cross-reactivity, verify detection in mouse-derived tissue or cell lines.
• When performing ELISA, use the immunizing phospho-peptide as a positive control and appropriate non-phosphorylated peptide as a negative control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with streamlined access to premium IVD raw materials, from concept to clinic. For transcription factor and phospho-specific antibody needs, our portfolio includes validated antibody pairs, optimized monoclonal and polyclonal antibodies, and ancillary reagents for bulk procurement. Backed by a global sourcing network, we readily accommodate rare‑target and custom‑development inquiries to accelerate your assay development.