Phospho-ATF-2 (Thr69/71)/ATF-7 (Thr51/53) Rabbit Polyclonal Antibody for WB, ELISA - P15336/P17544

Core Product Specifications and Parameters

Biological Background: ATF-2/ATF-7 Function and Localization

• ATF-2, also known as CREB-2 or CRE-BP1, is a member of the ATF/CREB family of basic region-leucine zipper (bZIP) transcription factors. It regulates gene expression by binding to CRE (5'-TGACGTCA-3') or AP-1 (5'-TGACTCA-3') consensus sequences, playing roles in anti-apoptosis, cell growth, and DNA damage response.
• The protein exhibits dual functions in cell survival and death: in the nucleus it promotes transcription, while in the cytoplasm it interacts with mitochondrial complexes (HK1/VDAC1) to induce mitochondrial leakage and cell death.
• ATF-2 is ubiquitously expressed with higher abundance in the brain, and localizes to the nucleus, cytoplasm, and mitochondrial outer membrane.
• It possesses intrinsic histone acetyltransferase (HAT) activity, acetylating histones H2B and H4 in vitro. Related references: PMID:10821277
• In response to DNA damage, ATM-mediated phosphorylation of ATF-2 at Thr69/71 contributes to S-phase checkpoint control and the recruitment of the MRN complex to ionizing radiation-induced foci.
• ATF-2 can act as either an oncogene or tumor suppressor depending on the cellular context, highlighting its complex role in cancer biology.
• This antibody also recognizes the homologous phosphorylation site in ATF-7 (Thr51/53), a related bZIP transcription factor that shares functional redundancy with ATF-2 in some signaling pathways.

Experimental Guidance and Technical Tips

• Since the antibody targets phosphorylation-dependent epitopes, ensure that samples are prepared with phosphatase inhibitors to preserve phospho-signals.
• For Western blot, consider using non-phospho-specific antibodies as controls to verify phosphorylation-specific detection.
• Cross-reactivity with the homologous ATF-7 phosphorylation site (Thr51/53) is expected based on sequence similarity; validate specificity in your experimental system using appropriate peptide competition or knockout models.
• The antibody is suitable for ELISA; appropriate blocking and washing conditions should be optimized for phospho-peptide coating.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with a comprehensive range of IVD raw materials, including validated phospho-specific and total antibodies, recombinant proteins, and ancillary reagents. This anti-phospho-ATF-2/ATF-7 antibody is part of our transcription factor and cell signaling portfolio, supporting biomarker discovery and assay development. With one-stop sourcing and technical support, we help streamline your workflow from concept to clinic.