Phospho-ASK1-T845 Rabbit mAb for WB, DB, ELISA - O35099

Core Product Specifications and Parameters

Biological Background: ASK1 Function and Localization

• Mitogen-activated protein kinase kinase kinase 5 (MAP3K5), also designated Apoptosis signal-regulating kinase 1 (ASK-1) or MEK kinase 5 (MEKK5), is a serine/threonine kinase encoded by the Map3k5 gene in mouse.
• Functions as a core component of the MAP kinase signal transduction cascade, translating environmental cues into cellular decisions involving differentiation, survival, and apoptosis.
• Plays a critical role in the mitochondria-dependent apoptosis pathway through caspase activation, responding to various stress stimuli.
• Essential for innate immune responses; mediates signaling triggered by oxidative stress, TNF, and LPS, leading to activation of the MKK4/MKK7–JNK and MKK3/MKK6–p38 MAPK pathways.
• Localizes predominantly to the cytoplasm and endoplasmic reticulum.
• Expressed broadly in adult mouse tissues, including heart, brain, lung, liver, and kidney.
• The protein is subject to phosphorylation, methylation, and ubiquitination; Thr845 phosphorylation is a key activation event in response to stress.

Experimental Guidance and Technical Tips

• The antibody is generated against a synthetic phospho-peptide spanning Thr845 of mouse ASK1, providing high specificity for the activated form of this kinase.
• In western blotting, a prominent band at approximately 155 kDa is expected; pre‑stained molecular weight markers should be included to confirm band size.
• For robust detection of endogenous phospho‑ASK1, stimulate cells with an appropriate stressor (e.g., oxidative stress or TNFα) and include phosphatase inhibitors in lysis and reaction buffers.
• The product is suitable for dot‑blot and ELISA applications; when using these formats, optimize blocking and washing conditions to minimize background.
• As with any phosphorylation‑specific antibody, confirm signal specificity by treating samples with λ‑phosphatase to verify loss of immunoreactivity.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers and research laboratories with a streamlined supply chain for high‑quality IVD raw materials. This anti‑phospho‑ASK1 antibody supports projects in apoptosis, stress signaling, and innate immunity research. As your strategic sourcing partner, CamelBio provides validated monoclonal/polyclonal antibodies, pre‑matched antibody pairs, bulk ancillary reagents, and tailored procurement for rare‑target reagents—helping you accelerate from discovery to clinical application.