Phospho-AMPKα1-S485 Rabbit Polyclonal Antibody for WB, ELISA - Q13131

Core Product Specifications and Parameters

Biological Background: AMPKα1 Function and Localization

• AMPKα1, encoded by the PRKAA1 gene, serves as the catalytic subunit of the AMP-activated protein kinase (AMPK), a cellular energy sensor. Upon reduced ATP levels, AMPK restores energy balance by activating catabolic pathways and suppressing biosynthetic processes.
  Related references: PMID:17307971, PMID:17712357

• In lipid metabolism, AMPKα1 phosphorylates and inactivates key enzymes including acetyl-CoA carboxylase (ACACA/ACACB) and HMG-CoA reductase (HMGCR), thereby inhibiting fatty acid and cholesterol synthesis. It also promotes lipolysis via phosphorylation of CHKA (CHKalpha2).
  Related references: PMID:17307971, PMID:17712357, PMID:34077757

• For glucose homeostasis, AMPKα1 stimulates glucose uptake by mediating GLUT4 translocation and regulates gluconeogenesis through phosphorylation of transcription coactivators such as CRTC2/TORC2 and FOXO3.
  Related references: PMID:11518699, PMID:11554766

• AMPKα1 negatively regulates mTORC1 signaling by phosphorylating TSC2 and RPTOR, leading to suppression of cell growth and proliferation under nutrient deprivation.
  Related references: PMID:14651849, PMID:18439900, PMID:20160076

• It promotes autophagy by phosphorylating and activating the ULK1 kinase complex and WDR45/WIPI4, critical for autophagosome formation.
  Related references: PMID:21205641, PMID:28561066

• AMPKα1 localizes to the cytoplasm, nucleus, and late endosomes, reflecting its roles in signal transduction, transcriptional regulation, and vesicle trafficking.

• AMPKα1 is a phosphoprotein; its activation loop phosphorylation at Thr172 is essential for kinase activity. This antibody specifically detects phosphorylation at serine 485, a site implicated in AMPK inhibition under certain conditions.

• The protein is also known as Acetyl-CoA carboxylase kinase, Hydroxymethylglutaryl-CoA reductase kinase, and Tau-protein kinase PRKAA1. It participates in additional processes including circadian rhythm, Wnt signaling, and host-virus interactions.

Experimental Guidance and Technical Tips

• This rabbit polyclonal antibody was raised against a synthetic phosphorylated peptide corresponding to Ser485 of human PRKAA1; it is expected to detect endogenous AMPKα1 phosphorylated at S485 in human, mouse, and rat samples by western blotting.
• For WB applications, consider using phosphatase inhibitor cocktails during lysis to preserve phosphorylation, and validate band specificity using phosphatase-treated controls.
• For ELISA, pairing with a total AMPKα1 capture antibody may enable sandwich detection of phospho-AMPKα1; optimize coating and detection conditions for your assay.
• Because S485 phosphorylation dynamics may vary across cell types and stimuli, confirm antibody performance in your specific experimental model.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we specialize in bridging the gap between IVD raw-material demand and supply, supporting your projects from early development to commercialization. This Phospho-AMPKα1-S485 antibody facilitates research into energy metabolism, AMPK signaling, and related therapeutic areas. Beyond single reagents, we offer validated antibody pairs, optimized monoclonal and polyclonal antibodies, bulk ancillary reagents, and tailored sourcing for rare targets—all designed to streamline your assay development pipeline.