Phospho-ALK-Y1096 Rabbit pAb for WB, ELISA - Q9UM73

Core Product Specifications and Parameters

Biological Background: ALK Function and Localization

• ALK tyrosine kinase receptor, also known as CD246, is a neuronal receptor tyrosine kinase essentially and transiently expressed in specific regions of the central and peripheral nervous systems, playing an important role in the genesis and differentiation of the nervous system. Related references: PMID:11121404, PMID:11387242, PMID:16317043
• Functions as a key thinness protein involved in resistance to weight gain; in hypothalamic neurons, controls energy expenditure by negatively regulating white adipose tissue lipolysis and sympathetic tone to fine-tune energy homeostasis.
• Upon ligand binding (e.g., ALKAL2), ALK activates downstream signaling including the MAPK pathway, leading to cell proliferation and anti-apoptotic responses. Related references: PMID:30061385, PMID:33411331, PMID:34646012, PMID:34819673
• Phosphorylates almost exclusively at the first tyrosine of the Y-x-x-x-Y-Y motif; the phospho-Y1096 site corresponds to this activation loop motif and is crucial for kinase activity and downstream signaling. Related references: PMID:15226403, PMID:16878150
• Induces tyrosine phosphorylation of substrates including CBL, FRS2, IRS1, SHC1, and MAPKs ERK1/2, contributing to NF-kappa-B activation and cell survival signaling. Related references: PMID:15226403, PMID:16878150
• Subcellular localization at the cell membrane; expressed in brain, central nervous system, small intestine, and testis, but not in normal lymphoid cells.
• Associated with chromosomal rearrangements and disease variants, relevant in neuroblastoma and anaplastic large cell lymphoma.

Experimental Guidance and Technical Tips

• For Western blot (WB), use phosphatase inhibitors during sample preparation to preserve phosphorylation signals. Consider treating cells with ALK ligands (e.g., ALKAL2) or phosphatase inhibitors to enhance pY1096 detection.
• In ELISA applications, validate with phosphorylated peptide coating and compare to non-phosphorylated controls to confirm specificity.
• Given the large molecular weight (~176 kDa), use appropriate gel percentage (e.g., 6–8%) and extended transfer times (e.g., overnight at 30 V) for efficient transfer.
• The antibody is validated for human samples; cross-reactivity with mouse or rat may be limited—validate in the relevant species model.

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