Phospho-AKT1S1/PRAS40-T246 Rabbit Monoclonal Antibody for WB, ELISA - Q96B36

Core Product Specifications and Parameters

Biological Background: AKT1S1/PRAS40 Function and Localization

• Proline-rich AKT1 substrate 1 (AKT1S1), also commonly referred to as PRAS40 (40 kDa proline-rich AKT substrate), is encoded by the AKT1S1 gene in humans.
• AKT1S1 acts as a key negative regulator of the mechanistic target of rapamycin complex 1 (mTORC1), a central nutrient sensor that promotes cell growth and biomass accumulation. It directly inhibits mTORC1 activity by blocking the substrate-recruitment site of the MTOR kinase. Related references: PMID:17277771, PMID:17386266, PMID:17510057, PMID:29236692
• In response to insulin and nutrients, AKT1S1 dissociates from mTORC1, relieving inhibition and allowing mTORC1 signaling to proceed, a process tightly linked to its phosphorylation status and association with 14-3-3 proteins. Related references: PMID:17386266, PMID:18372248, PMID:16174443
• Predominantly localized in the cytoplasm and cytosol, AKT1S1 is widely expressed across tissues, with highest levels observed in the liver and heart. Notably, its expression is often elevated in cancer cell lines such as A-549 and HeLa compared to normal cell lines.
• The protein undergoes multiple post-translational modifications, including phosphorylation at several residues and methylation, and is subject to alternative splicing, contributing to its functional regulation.
• Beyond metabolic signaling, AKT1S1 may also play a role in nerve growth factor-mediated neuroprotection, though this function is primarily inferred from similarity to other species.

Experimental Guidance and Technical Tips

• This antibody was generated using a synthetic phosphopeptide corresponding to the Thr246 phosphorylation site. To maintain phospho-specific detection, consider using phosphatase inhibitors during cell/tissue lysis and immunoprecipitation steps.
• For western blotting (WB), a protein band of approximately 27 kDa is expected based on the calculated molecular weight of AKT1S1.
• When performing ELISA, appropriate peptide blocking and phosphorylated/non-phosphorylated peptide controls may help confirm specificity for the T246-phosphorylated form.
• Given the ubiquitous expression and conditional phosphorylation of AKT1S1, validating antibody performance in the relevant sample system (e.g., insulin-stimulated versus starved cells) is recommended.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive one-stop partner for IVD raw material sourcing, supporting diagnostic manufacturers and research institutions. This Phospho-AKT1S1-T246 antibody targets a critical node in mTOR signaling and cancer metabolism, and CamelBio can assist with fulfilling broader project needs—from validated antibody pairs and optimized monoclonal/polyclonal antibodies to bulk ancillary reagents and custom rare-target procurement. Our supply solutions aim to streamline your research and diagnostic development workflows with reliable and timely delivery.