Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit Polyclonal Antibody for WB, ELISA - P31749 / P31751 / Q9Y243

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is one of three closely related serine/threonine-protein kinases (AKT1, AKT2, AKT3) that regulate metabolism, proliferation, cell survival, growth, and angiogenesis. Related references: PMID:11882383 PMID:15526160 PMID:15861136
• AKT1 phosphorylates over 100 downstream substrates, mediating the effects of growth factors such as insulin, IGF1, PDGF, and EGF. Related references: PMID:21432781 PMID:12176338 PMID:12964941
• In insulin signaling, AKT1 promotes glucose uptake by triggering SLC2A4/GLUT4 translocation and phosphorylates GSK3A/B to stimulate glycogen synthesis.
• AKT1 regulates cell survival by phosphorylating MAP3K5/ASK1 at Ser-83, inhibiting oxidative stress-induced apoptosis. Related references: PMID:11154276
• AKT1 activates mTORC1 by phosphorylating TSC2 at Ser-939 and Thr-1462, thereby promoting protein synthesis and cell growth. Related references: PMID:12150915 PMID:12172553
• AKT1 phosphorylates FOXO transcription factors (FOXO1, FOXO3, FOXO4), leading to 14-3-3 binding and cytoplasmic sequestration, promoting cell proliferation and survival. Related references: PMID:10358075
• Subcellular localization includes the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, with regulated translocation depending on stimuli.
• Aberrant AKT1 activation is associated with cancer; its expression increases in prostate and breast cancers, and Tyr-176 phosphorylation correlates with malignancy stages.
• Phosphorylation at Thr-308 in the activation loop is required for AKT1 kinase activity, and this site is conserved in AKT2 (Thr-309) and AKT3 (Thr-305).

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Thr-308 of human AKT1; the antibody is expected to recognize the phosphorylated forms of AKT1 (p-T308), AKT2 (p-T309), and AKT3 (p-T305).
• For Western blot, a band around 48–55 kDa is expected depending on the isoform and post-translational modifications; consider using phosphatase inhibitors during sample preparation to preserve phosphorylations.
• Validate antibody specificity by treating samples with lambda phosphatase or using non-phosphorylated peptide blocking.
• For ELISA, the antibody can be used to detect phosphorylated AKT in cell lysates; titrate antibody and use appropriate phospho- and total protein controls.
• Cross-reactivity is indicated for human and mouse; additional species may need empirical validation.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides comprehensive IVD raw material solutions for diagnostic manufacturers and research labs. This phospho-AKT (T308/T309/T305) antibody supports oncology and cell signaling research, detecting key activation markers in the PI3K/AKT pathway. Our platform offers validated antibody pairs, bulk reagents, and technical consultation to streamline your assay development from bench to bedside.