Phospho-AKT1-T308+AKT2-T309+AKT3-T305 Rabbit pAb - P31749 / P31751 / Q9Y243

Core Product Specifications and Parameters

Biological Background: AKT1 (RAC-alpha serine/threonine-protein kinase) Function and Localization

• AKT1 (PKBα, RAC-PK-alpha) is a ubiquitously expressed serine/threonine kinase that, together with AKT2 and AKT3, forms the AKT kinase family central to PI3K signaling, governing metabolism, proliferation, survival, growth, and angiogenesis. Related references: PMID:11882383 PMID:15526160 PMID:15861136
• Through phosphorylation of numerous substrates, AKT1 mediates insulin-induced glucose uptake (TBC1D4) and glycogen synthesis (GSK3A/GSK3B). Related references: PMID:11994271
• It promotes cell survival by phosphorylating MAP3K5/ASK1 at Ser-83, inhibiting apoptosis, and drives mTORC1 signaling via TSC2 and DEPDC5 phosphorylation. Related references: PMID:11154276 PMID:12150915 PMID:12172553 PMID:31548394 PMID:33594058
• AKT1 regulates transcription factors FOXO1/3/4 and CREB1, influencing pro-survival gene expression and NF-κB-dependent transcription. Related references: PMID:10358075 PMID:9829964
• Subcellularly, AKT1 shuttles between the cytoplasm, nucleus, cell membrane, and mitochondria, where it modulates mitochondrial calcium uptake and other organelle-specific functions.
• Key activating phosphorylations occur at Thr-308 (or equivalent Thr-309 in AKT2, Thr-305 in AKT3) in the activation loop and Ser-473 in the hydrophobic motif, triggered by growth factor stimulation.
• AKT1 is frequently dysregulated in cancers (prostate, breast) and acts as a proto-oncogene, with elevated phosphorylation levels correlating with disease progression.

Experimental Guidance and Technical Tips

• Use phosphatase inhibitors during sample preparation to preserve the phosphorylation signal; validate phospho-specificity by comparing with total AKT antibodies or by phosphatase treatment.
• The immunogen is a synthetic phosphopeptide around Thr-308 of human AKT1; the antibody is expected to detect phosphorylated AKT1, AKT2, and AKT3 at equivalent sites when tested in appropriate species.
• For Western blot, expect multiple bands corresponding to the various AKT isoforms (~48-55 kDa); confirm band identity with isoform-specific controls or siRNA if needed.
• For ELISA, consider a paired phospho-specific/total AKT antibody setup to normalize for protein loading.
• Optimize primary antibody dilution for your specific sample type; suggested starting dilutions for WB are typically 1:500-1:2000, but must be empirically determined.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to a comprehensive portfolio of IVD raw materials, including validated phospho-specific antibodies like this AKT1/2/3 phospho-T308/T309/T305 rabbit pAb. Whether you require bulk quantities of mono- or polyclonal antibodies, matched antibody pairs, or ancillary reagents for phospho-signaling pathway analysis, our one-stop sourcing model covers every step from concept to clinical application. For challenging targets within kinase signaling or oncology research, CamelBio’s expert team can source or develop custom raw materials to meet your specific assay requirements.