Phospho-AKT1-T308 Rabbit pAb - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is a member of the AKT serine/threonine kinase family that mediates diverse cellular processes, including metabolism, proliferation, survival, growth, and angiogenesis by phosphorylating numerous downstream targets. Related references: PMID:11882383 PMID:15526160 PMID:15861136 PMID:21432781

• It plays a central role in insulin signaling by promoting glucose uptake via SLC2A4/GLUT4 translocation and regulating glycogen synthesis through inhibitory phosphorylation of GSK3A (Ser-21) and GSK3B (Ser-9).

• AKT1 promotes protein synthesis and cell growth by phosphorylating TSC2 at Ser-939 and Thr-1462, thereby activating the mTORC1 pathway. Related references: PMID:12150915 PMID:12172553

• It regulates cell survival by inhibiting pro-apoptotic factors: phosphorylating MAP3K5/ASK1 at Ser-83 to suppress oxidative stress-induced apoptosis and phosphorylating BAD to inactivate its pro-apoptotic function. Related references: PMID:11154276 PMID:10926925

• AKT1 phosphorylates FOXO transcription factors (FOXO1 at Thr-24, Ser-256, Ser-319; FOXO3 and FOXO4 at equivalent sites), leading to their cytoplasmic retention and inactivation, which promotes cell proliferation. Related references: PMID:10358075

• Subcellularly, AKT1 localizes to the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, consistent with its multifaceted signaling roles.

• AKT1 is widely expressed in human tissues, with protein levels increased in prostate and breast cancers, particularly the Tyr-176 phosphorylated form showing elevated expression during breast cancer progression.

Experimental Guidance and Technical Tips

• For western blotting, consider using insulin-stimulated or growth factor-treated cell lysates as positive controls, and always include phosphatase inhibitors during sample preparation to preserve phosphorylation at Thr308.
• Validate specificity by comparing signal with a total AKT1 antibody, and test the antibody in the relevant model system (human, mouse, or rat) to confirm expected band at ~56 kDa.
• For ELISA applications, verify detection of the phosphorylated peptide versus the non-phosphorylated counterpart, and optimize coating concentrations and antibody dilutions empirically.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers a comprehensive portfolio of IVD raw materials, including validated antibody pairs, optimized monoclonal and polyclonal antibodies, and bulk ancillary reagents, to accelerate your AKT1-related signaling and oncology research. We also provide rare target raw-material sourcing and technical consulting to support your entire assay development journey—from concept to commercialization.