Phospho-AKT1-S124 Rabbit Monoclonal Antibody for WB and ELISA - P31749

Core Product Specifications and Parameters

Biological Background: AKT1 Function and Localization

• AKT1 is a serine/threonine-protein kinase belonging to the AKT family (AKT1, AKT2, AKT3) that regulates key processes including metabolism, proliferation, cell survival, growth, and angiogenesis (PubMed:11882383, PubMed:15526160, PubMed:15861136). Related references: PMID:11882383 PMID:15526160 PMID:15861136
• It signals downstream of PI3K to mediate effects of insulin and growth factors, promoting glucose uptake (SLC2A4/GLUT4), glycogen synthesis (via GSK3 inhibition), and protein synthesis (via mTORC1 activation) (PubMed:11994271, PubMed:12150915, PubMed:12172553). Related references: PMID:11994271 PMID:12150915 PMID:12172553
• AKT1 regulates cell survival by phosphorylating and inhibiting pro-apoptotic factors: it decreases MAP3K5/ASK1 activity (PubMed:11154276), stimulates BAD (PubMed:10926925), and activates NF-kappa-B transcription of BCL2/MCL1 (PubMed:9829964). Related references: PMID:11154276 PMID:10926925 PMID:9829964
• Phosphorylation of FOXO transcription factors (FOXO1, FOXO3, FOXO4) by AKT1 leads to 14-3-3 binding and cytoplasmic retention, thus inhibiting transcription of pro-apoptotic and cell-cycle arrest genes (PubMed:10358075). Related references: PMID:10358075
• Localized to the cytoplasm, nucleus, cell membrane, and mitochondrial intermembrane space, AKT1 dynamically translocates in response to stimuli; in mitochondria, it modulates calcium uptake and TMEM175 channel activity, independent of its kinase function (PubMed:32228865, PubMed:30504268).
• Expressed ubiquitously in human tissues, AKT1 is frequently hyperactivated in cancers; elevated phospho-Tyr176 AKT1 correlates with breast cancer progression from normal to metastatic stages.

Experimental Guidance and Technical Tips

• To ensure phospho-specificity, compare signals from lysates treated with and without lambda phosphatase, or perform peptide competition assays with the immunogen peptide and its non-phosphorylated counterpart.
• For Western blotting, lysates should be prepared with phosphatase inhibitors; the expected molecular weight is approximately 56 kDa; a loading control such as total AKT1 or GAPDH is recommended.
• In ELISA applications, use the synthetic phospho-peptide as a standard to generate a calibration curve for semi-quantitative analysis of phosphorylated AKT1-S124 in biological samples.
• Activation of AKT1 phosphorylation can be verified in insulin- or growth factor-stimulated cell lines; consider using inhibitors of PI3K (e.g., LY294002) to confirm pathway dependency.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a specialized supplier of IVD raw materials, offering comprehensive solutions for diagnostic development. This anti-phospho-AKT1-S124 rabbit monoclonal antibody supports research into the PI3K/AKT pathway relevant to oncology and metabolic diseases. Beyond single antibodies, we provide validated antibody pairs, bulk ancillary reagents, and rare target sourcing to streamline your assay pipeline from concept to clinical application.