Phospho-ACLY-S455 Rabbit pAb for WB, IHC-P, IP, ELISA - P53396

Core Product Specifications and Parameters

Biological Background: ATP-citrate synthase Function and Localization

• ATP-citrate synthase (ACLY), also known as ATP-citrate lyase (ACL) or citrate cleavage enzyme, is encoded by the ACLY gene in humans.
• The enzyme catalyzes the ATP-dependent cleavage of citrate into oxaloacetate and acetyl-CoA, providing a key substrate for lipogenesis and other metabolic pathways.
• ACLY is a central metabolic enzyme linking carbohydrate catabolism to lipid biosynthesis, playing a critical role in overall cellular metabolism.
• The protein localizes predominantly in the cytoplasm/cytosol, as indicated by subcellular localization data.
• ACLY is subject to various post-translational modifications including phosphorylation (e.g., at Ser455), acetylation, and ubiquitination, which regulate its activity and stability.
• Alternative splicing of the ACLY gene results in multiple isoforms, contributing to functional diversity.
• The enzyme requires metal ions (magnesium) and ATP for its catalytic activity, as reflected in its nucleotide-binding and metal-binding properties.

Experimental Guidance and Technical Tips

• This antibody was raised against a phosphopeptide corresponding to Ser455 of human ACLY; therefore, it recognizes the phosphorylated form of the protein. Consider confirming specificity by treating samples with lambda phosphatase before analysis.
• For Western blot, the expected molecular weight is approximately 121 kDa. Use appropriate gel percentages and loading controls to ensure proper resolution.
• In immunohistochemistry (paraffin), antigen retrieval may be required to expose the epitope; optimize citrate or EDTA-based methods for your tissue type.
• For immunoprecipitation, perform a pilot experiment to determine optimal antibody-to-lysate ratios and include a non-specific IgG control.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to high-quality IVD raw materials, including validated phospho-specific antibodies like this anti-ACLY pS455 pAb. Our portfolio covers a wide range of metabolic targets, supported by bulk ancillary reagents and rare-target raw-material sourcing to accelerate your assay development from concept to clinic.