Phospho-ACC1/ACC2-S79 Rabbit PolymAb® for WB, IF/ICC, ELISA - Q13085 / O00763

Core Product Specifications and Parameters

Biological Background: Acetyl-CoA Carboxylase 1/2 Function and Localization

• ACC1 (acetyl-CoA carboxylase 1, encoded by ACACA) is a biotin-dependent multifunctional enzyme that catalyzes the ATP-dependent carboxylation of acetyl-CoA to malonyl-CoA, the first and rate‑limiting step of de novo fatty acid biosynthesis. Related references: PMID:20457939 PMID:20952656 PMID:29899443
• ACC2 (acetyl-CoA carboxylase 2, encoded by ACACB) is a homologous isoform that primarily regulates fatty acid oxidation; the antibody recognizes a conserved phosphorylation site common to both ACC1 and ACC2.
• The enzyme localizes predominantly to the cytoplasm / cytosol.
• ACC1 is expressed in brain, placenta, skeletal muscle, renal, pancreatic and adipose tissues; it is present at low levels in lung and is not detected in liver.
• As a phosphoprotein, its activity is modulated by phosphorylation at key residues such as Ser79 (ACC1 numbering), linking cellular energy status to lipid metabolism.
• The protein requires biotin as a cofactor and binds ATP, Mg²⁺ or Mn²⁺; it carries a biotin carboxyl carrier domain essential for its function.
• Additional post‑translational modifications include acetylation, and the enzyme is subject to allosteric regulation.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide corresponding to residues around Ser79; phosphatase inhibitors should therefore be included during sample preparation to preserve the phosphorylation signal.
• For Western blotting, note the large predicted molecular weight (~265kDa); consider using low‑percentage SDS‑PAGE gels, extended transfer times, and appropriate molecular weight markers.
• In IF/ICC, optimized fixation and permeabilization conditions may be necessary to achieve robust cytoplasmic staining.
• When performing ELISA, verify phospho‑specificity by comparing binding to phosphorylated versus non‑phosphorylated peptides or after phosphatase treatment of samples.

CamelBio: Your One‑Stop Sourcing Bridge

CamelBio empowers diagnostic manufacturers, laboratories, and research institutes with a one‑stop sourcing solution for IVD raw materials, including validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. This phospho‑specific ACC1/ACC2 antibody supports investigations into fatty acid metabolism and metabolic disorders. Contact us for custom bulk orders, rare‑target raw‑material sourcing, and comprehensive technical consulting—delivering seamless support from concept to clinic.