Phospho-ACC1 (Ser79) Rabbit Polyclonal Antibody for WB, ELISA - Q13085

Core Product Specifications and Parameters

Biological Background: ACC1 Function and Localization

• Acetyl-CoA carboxylase 1 (ACC1), encoded by the ACACA gene, is a cytosolic enzyme that catalyzes the carboxylation of acetyl-CoA to malonyl-CoA, the first and rate-limiting step of de novo fatty acid biosynthesis. This two-step reaction involves the ATP-dependent carboxylation of the biotin cofactor on the biotin carboxyl carrier (BCC) domain, followed by transfer of the carboxyl group to acetyl-CoA. Related references: PMID:20457939, PMID:20952656, PMID:29899443
• ACC1 is a multifunctional enzyme with multiple domains, including biotin carboxylase, biotin carboxyl carrier, and carboxyltransferase, and requires magnesium or manganese as cofactors.
• The enzyme is subject to multiple post-translational modifications, including acetylation and phosphorylation, which modulate its activity and stability.
• ACC1 localizes to the cytoplasm/cytosol.
• Tissue expression includes brain, placenta, skeletal muscle, renal, pancreatic, and adipose tissues; it is expressed at low levels in the lung and not detected in the liver.
• Alternative promoter usage generates different isoforms; the enzyme is allosterically regulated by citrate and palmitoyl-CoA.

Experimental Guidance and Technical Tips

• The immunogen is a synthetic phosphorylated peptide around Ser79; specificity should be confirmed by competition with non-phosphorylated peptide or phosphatase treatment in WB.
• For WB, a 266 kDa band is expected; consider using gradient gels and extended transfer times due to the high molecular weight.
• For ELISA, the antibody may be used for detection of phosphorylated ACC1 in cell lysates; validate in the relevant sample system.
• Cross-reactivity with human, mouse, and rat ACC1 is reported; verify target sequence homology for other species.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research labs with comprehensive access to IVD raw materials, including validated antibodies, antigen pairs, and ancillary reagents for metabolic and signaling pathways. This phospho-ACC1 (Ser79) antibody supports fatty acid metabolism and obesity-related research, with CamelBio offering one-stop sourcing from discovery to clinic. For rare targets or bulk orders, our team bridges supply gaps with tailored procurement and technical consultation.