Phospho-α-Synuclein-Y133 Rabbit pAb for WB, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Highly expressed in brain, particularly in presynaptic terminals in the central nervous system.
• Regulates synaptic vesicle trafficking and neurotransmitter release. Related references: PMID:20798282, PMID:26442590, PMID:28288128
• Acts as a molecular chaperone assisting in the folding of SNARE proteins, sustaining SNARE-complex assembly during aging. Related references: PMID:20798282
• Functions as a monomer in synaptic vesicle exocytosis by enhancing vesicle priming, fusion, and dilation of exocytotic fusion pores; increases local Ca2+ release to enhance ATP-induced exocytosis. Related references: PMID:28288128, PMID:30404828
• Modulates dopamine neurotransmission by associating with dopamine transporter (DAT1) and regulating its activity. Related references: PMID:26442590
• Subcellular localization includes cytoplasm, membrane, nucleus, synapse, and secreted; also found in cell projections such as axons.
• Keywords indicate roles in amyloid formation, neurodegeneration (Alzheimer and Parkinson disease), and post-translational modifications including phosphorylation at Tyr133.

Experimental Guidance and Technical Tips

• For Western blot, consider adding phosphatase inhibitors to the lysis buffer to preserve the Y133 phosphorylation state.
• Validate antibody specificity using a non-phosphorylated peptide or protein as a negative control in your relevant sample system.
• For ELISA, optimize coating conditions with the phosphorylated peptide or full-length protein; confirm specificity with non-phosphorylated controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio simplifies your IVD development with one-stop access to high-quality raw materials. For alpha-synuclein and neurodegenerative disease biomarkers, we offer validated phospho-specific antibodies, optimized monoclonal and polyclonal options, and bulk ancillary reagents. From concept to clinic, CamelBio bridges your sourcing needs efficiently.