Phospho-α-Synuclein-S129 Rabbit Polyclonal Antibody for WB, ELISA - P37840

Core Product Specifications and Parameters

Biological Background: Alpha-synuclein Function and Localization

• Alpha-synuclein (SNCA) is a 14kDa neuronal protein also known as NACP, linked to Parkinson's disease (PARK1, PARK4) and Alzheimer's.
• Regulates synaptic vesicle trafficking and neurotransmitter release, enhancing ATP-induced exocytosis by increasing local Ca2+ release from microdomains. Related references: PMID:28288128, PMID:30404828
• Acts as a molecular chaperone in its multimeric membrane-bound state, assisting SNARE-complex assembly with DNAJC5 to sustain synaptic function during aging. Related references: PMID:20798282
• Modulates dopamine neurotransmission by associating with dopamine transporter DAT1/SLC6A3. Related references: PMID:26442590
• Phosphorylation at serine 129 is a pathological hallmark; localizes to cytoplasm, membrane, nucleus, synapse, and can be secreted, with high expression in brain presynaptic terminals.

Experimental Guidance and Technical Tips

• This antibody was raised against a synthetic phospho-peptide surrounding Ser129 and is expected to specifically recognize the phosphorylated form of alpha-synuclein.
• For Western blot, consider low-voltage transfer with phospho-enhancing buffers and validate specificity by phosphatase treatment or comparison with total alpha-synuclein blots.
• In ELISA, use as a capture or detection antibody for quantifying phospho-alpha-synuclein in biological fluids; validate with phospho-peptide standards and appropriate negative controls.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is a one-stop IVD raw material provider, offering validated antibody pairs, bulk monoclonal/polyclonal antibodies, and ancillary reagents for neurodegenerative disease research. For alpha-synuclein studies, we supply phosphorylation-specific antibodies like this Rabbit pAb, rare target sourcing, and technical consulting to accelerate your assay development from concept to clinic.