PHF21A Rabbit Polyclonal Antibody for WB and ELISA - Q96BD5

Core Product Specifications and Parameters

Biological Background: PHF21A Function and Localization

• PHF21A, also known as BHC80 or BRAF35-HDAC complex protein BHC80, is a PHD finger protein.
• It is a component of the BHC (BRAF35/HDAC) corepressor complex, which represses transcription of neuron-specific genes in non-neuronal cells.
• The BHC complex is recruited to RE1/NRSE sites by REST and modifies chromatin via histone deacetylation and demethylation.
• PHF21A may function as a scaffold within the BHC complex and inhibits KDM1A-mediated demethylation of histone H3 'Lys-4' in vitro, suggesting a regulatory role in demethylation.
• The protein localizes to the nucleus.
• PHF21A contains a PHD finger domain, which likely mediates DNA-binding and zinc-binding, consistent with its function as a chromatin regulator.
• Post-translational modifications of PHF21A include phosphorylation and ubiquitination.
• PHF21A is highly expressed in the brain, with lower expression in heart, kidney, liver, lung, and skeletal muscle, and is abundant in fetal brain.

Experimental Guidance and Technical Tips

• The immunogen sequence (residues 461-680) corresponds to the C-terminal region of human PHF21A; when using mouse or rat samples, consider verifying epitope conservation as cross-reactivity is expected.
• For Western blotting, expect a band at approximately 75 kDa under standard conditions; validate with appropriate molecular weight markers.
• In ELISA applications, confirm specificity using recombinant PHF21A or BHC complex components.
• Since PHF21A is a nuclear protein, efficient nuclear extraction may be required for optimal detection in cell or tissue lysates.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio acts as a one-stop sourcing bridge for IVD raw materials, connecting diagnostic manufacturers and research labs with rigorously characterized antibodies such as this anti-PHF21A polyclonal antibody. We support epigenetic and neurodevelopment research programs by supplying validated antibody pairs, optimized monoclonal/polyclonal antibodies, bulk ancillary reagents, and rare-target raw-material sourcing. Contact us to explore tailored procurement solutions that advance your diagnostic pipeline from concept to clinic.