PHD3 Rabbit mAb for WB, IHC-P, IF/ICC, ELISA - Q9H6Z9

Core Product Specifications and Parameters

Biological Background: PHD3 Function and Localization

• Encoded by the EGLN3 gene, PHD3 (also designated HIFPH3/HIFP4H3) is an iron/2-oxoglutarate-dependent dioxygenase belonging to the EGLN family. It contains a prolyl hydroxylase domain and undergoes ubiquitination.
• Functions as a cellular oxygen sensor: under normoxia, hydroxylates specific proline residues in the oxygen-dependent degradation (ODD) domains of HIF1A and HIF2A, targeting them for VHL-mediated ubiquitination and proteasomal degradation. Under hypoxia, hydroxylation is attenuated, allowing HIFs to stabilize, translocate to the nucleus, and drive hypoxic gene expression. Prefers the CODD site. Related references: PMID:11595184 PMID:12181324
• Hydroxylates PKM in hypoxia to limit glycolysis, and regulates ADRB2 stability under normoxic conditions. Related references: PMID:21483450 PMID:21620138 PMID:19584355
• Acts in the DNA damage response by hydroxylating TELO2, facilitating ATR/CHK1/p53 pathway activation. Related references: PMID:22797300
• Inhibits ferroptosis through hydroxylation of GPX4, which prevents its chaperone-mediated autophagy-dependent degradation; this reaction is promoted by PSAT1-provided 2-oxoglutarate. Related references: PMID:40281343
• Regulates apoptosis: in cardiomyocytes, disrupts the BAX-BCL2 complex to counteract BCL2's anti-apoptotic effect; in neurons, mediates NGF-induced proapoptotic signaling via CASP3. Related references: PMID:20849813 PMID:16098468
• Essential for hypoxic regulation of neutrophilic inflammation. Related references: PMID:21317538
• Localizes to both the nucleus and cytoplasm. Widely expressed at low levels, with enrichment in adult heart, lung, and placenta; also found in fetal spleen, heart, skeletal muscle, and pancreatic acini/islet cells.

Experimental Guidance and Technical Tips

• This rabbit monoclonal antibody was raised against a synthetic peptide covering the N-terminal region (aa 1–100) of human PHD3.
• For Western blot (WB), expect a band at approximately 27 kDa; validate signals using lysates from tissues with known high PHD3 expression (e.g., heart, lung) and consider hypoxia-induced dynamic changes.
• In immunohistochemistry (IHC-P) and immunofluorescence (IF/ICC), anticipate nuclear and cytoplasmic staining; always include appropriate positive and negative controls.
• ELISA and other applications are reported but may require individual optimization.

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