PGK1 Rabbit Monoclonal Antibody for WB, ELISA - P00558

Core Product Specifications and Parameters

Biological Background: PGK1 Function and Localization

• PGK1 encodes phosphoglycerate kinase 1, a glycolytic enzyme that catalyzes the reversible conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, producing ATP. Related references: PMID:30323285, PMID:7391028
• In addition to its cytoplasmic role, PGK1 translocates to the mitochondrial matrix where it functions as a protein kinase, phosphorylating PDK1 to inhibit the pyruvate dehydrogenase complex, suppressing oxidative phosphorylation and promoting glycolysis. Related references: PMID:26942675, PMID:36849569
• PGK1 acts as a polymerase alpha cofactor (primer recognition protein), linking glycolysis to DNA replication. Related references: PMID:2324090
• It shows broad nucleotide substrate specificity, with ability to use both L- and D- forms of purine and pyrimidine nucleotides, though pyrimidine activity is lower. Related references: PMID:18463139
• PGK1 is mainly expressed in spermatogonia and localized to the sperm principal piece, suggesting a role in sperm motility. Related references: PMID:26677959
• Alternative splicing generates multiple isoforms; the protein undergoes acetylation, phosphorylation, and hydroxylation modifications.
• Disease-associated variants are linked to hereditary hemolytic anemia due to phosphoglycerate kinase deficiency.

Experimental Guidance and Technical Tips

• Based on the immunogen design (N-terminal peptide), this antibody is expected to recognize the N-terminal region, which may detect full-length PGK1 and N-terminal fragments. Validate in your experimental context.
• For Western blot, the expected molecular weight is approximately 45 kDa; however, post-translational modifications may cause slight shifts. Use appropriate positive controls (e.g., HeLa, HepG2 lysates) and validate in mouse/rat samples if cross-reactivity is desired.
• For ELISA, consider pairing with a detection antibody targeting a different epitope; optimize coating conditions and antibody titers for your specific assay requirements.
• Given PGK1's dual cytoplasmic and mitochondrial localization, subcellular fractionation may be useful to study compartment-specific functions.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research laboratories with streamlined access to high-quality IVD raw materials, from validated antibody pairs and optimized monoclonal antibodies to bulk ancillary reagents. Whether you require PGK1 antibodies for glycolysis-related assay development or need assistance sourcing rare-target reagents, our integrated supply chain supports your project from concept to clinic. Contact us to explore reliable, scalable solutions tailored to your workflow.