PFKFB3 Rabbit Monoclonal Antibody for WB, IHC-P, ELISA - Q16875

Core Product Specifications and Parameters

Biological Background: PFKFB3 Function and Localization

• PFKFB3 (6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 3) is a bifunctional enzyme that catalyzes both the synthesis and degradation of fructose-2,6-bisphosphate, a potent allosteric regulator of glycolysis.
• It is also known as renal carcinoma antigen NY-REN-56 and inducible phosphofructokinase-2 (iPFK-2).
• PFKFB3 belongs to the phosphofructokinase-2/fructose-2,6-bisphosphatase family, which includes four isozymes (PFKFB1-4) with tissue-specific expression; PFKFB3 is ubiquitously expressed.
• By controlling the intracellular concentration of fructose-2,6-bisphosphate, PFKFB3 plays a critical role in regulating glycolytic flux and energy metabolism.
• The enzyme is subject to alternative splicing, generating multiple isoforms (UniProt keyword: Alternative splicing).
• PFKFB3 possesses both kinase (transferase) and bisphosphatase (hydrolase) activities, classifying it as a multifunctional enzyme.
• Regulation occurs through phosphorylation (UniProt keyword: Phosphoprotein); additional keywords include 3D-structure, ATP-binding, Nucleotide-binding, and Reference proteome.

Experimental Guidance and Technical Tips

• The immunogen amino acids 400-500 lie in the C-terminal region, which may be conserved across species, supporting the reported cross-reactivity with human and mouse.
• For WB, expect a specific band at approximately 60 kDa; validate in relevant sample systems (human, mouse) using appropriate loading controls.
• For IHC-P, antigen retrieval may be necessary; consider heat-induced retrieval in citrate buffer (pH 6.0) or EDTA buffer (pH 9.0).
• As a monoclonal antibody, it offers high specificity; always include negative and positive controls in experiments.
• ELISA applications require optimization of coating antigen concentration and antibody dilution to achieve optimal signal-to-noise ratio.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, research laboratories, and institutes a comprehensive one-stop solution for IVD raw materials, including validated antibody pairs and optimized monoclonal/polyclonal antibodies. Our PFKFB3 Rabbit Monoclonal Antibody supports oncology and metabolism research, while our sourcing capabilities extend to bulk ancillary reagents and rare target raw-material procurement to accelerate your assay development.