PFKFB2 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - O60825

Core Product Specifications and Parameters

Biological Background: PFKFB2 Function and Localization

• PFKFB2 encodes the heart-type isozyme of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (6PF-2-K/Fru-2,6-P2ase), also known as PFK-2/FBPase-2.
• The enzyme bifunctionally catalyzes both the synthesis and degradation of fructose 2,6-bisphosphate, a key allosteric regulator of glycolysis and gluconeogenesis.
• Through alternative splicing, the PFKFB2 gene produces multiple isoforms that may exhibit tissue-specific regulatory properties.
• Predominantly expressed in heart tissue, where it plays a critical role in modulating cardiac glycolytic flux in response to metabolic demands.
• The protein contains both kinase and phosphatase domains, as well as nucleotide-binding and ATP-binding sites, enabling dynamic control of its enzymatic activities.
• Post-translational modifications such as phosphorylation and acetylation regulate its activity and subcellular localization.
• Subcellular localization studies indicate the enzyme is primarily cytosolic, though specific isoforms may localize to other compartments under certain conditions.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a C-terminal region (aa 446-505) of human PFKFB2, which may recognize multiple isoforms due to sequence homology.
• For Western blot, the predicted molecular weight is 58 kDa; a band around this size is expected in heart tissue lysates. Validate specificity in relevant positive and negative cell lines or tissues.
• For IF/ICC, consider permeabilization conditions and include appropriate controls to confirm localization.
• The antibody cross-reacts with mouse and rat, facilitating translational studies across species.
• Because it is a polyclonal antibody, lot-to-lot consistency should be verified in your assay system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers PFKFB2 antibodies as part of its comprehensive portfolio of IVD raw materials for metabolic and cardiac research. Our supply network provides validated antibody pairs, bulk ancillary reagents, and custom monoclonal/polyclonal antibodies to support diagnostic development. With a one-stop sourcing model, CamelBio bridges the gap from concept to clinic, ensuring timely access to rare and specialized reagents.