PFDN4 Rabbit Polyclonal Antibody for WB, IF/ICC, ELISA - Q9NQP4

Core Product Specifications and Parameters

Biological Background: Prefoldin Subunit 4 Function and Localization

• Prefoldin subunit 4 (PFDN4), also known as Protein C-1, is a component of the prefoldin complex that functions as a molecular chaperone.
• It binds specifically to cytosolic chaperonin (c-CPN) and transfers target proteins to it, facilitating proper protein folding in the crowded cellular environment.
• PFDN4 binds to nascent polypeptide chains and promotes correct folding by preventing aggregation and off-pathway interactions.
• According to UniProt, PFDN4 localizes to the nucleus, cytoplasm, and mitochondrion, indicating a broad subcellular distribution linked to its chaperone activity.
• Post-translational modifications include acetylation and phosphorylation, as noted in the keyword list, which may regulate its function or interactions.
• The protein has a molecular weight of approximately 15.3 kDa (UniProt) and is part of the reference proteome.

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein covering amino acids 1-134 of human PFDN4, which includes nearly the full-length protein. This design may enable recognition of native conformations and denatured forms, supporting Western blot (WB) detection.
• For WB, a 15 kDa band is expected; consider using appropriate gel percentage and transfer conditions for low molecular weight proteins.
• In IF/ICC, PFDN4 may localize to the nucleus, cytoplasm, and mitochondria; use appropriate counterstains and controls to confirm specific staining patterns.
• For ELISA, ensure the coating antigen maintains conformational epitopes if the antibody recognizes native structure; validate with recombinant PFDN4 protein as a standard.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers, laboratories, and research institutes streamlined access to high-quality IVD raw materials, including validated antibodies, antigens, and ancillary reagents. This PFDN4 polyclonal antibody supports research into chaperone biology and protein folding—if you require matched antibody pairs, bulk quantities, or custom sourcing for rare targets, our team is equipped to assist across the development pipeline.