PERK Rabbit Polyclonal Antibody for IF/ICC, ELISA - Q9NZJ5

Core Product Specifications and Parameters

Biological Background: PERK Function and Localization

• Protein identity and isoforms: PERK (EIF2AK3) is also known as PRKR-like endoplasmic reticulum kinase, pancreatic eIF2-alpha kinase, and protein tyrosine kinase EIF2AK3. It belongs to the eIF2α kinase family and is the primary sensor of ER stress.
• Unfolded protein response (UPR) kinase: PERK is a metabolic-stress sensing kinase that phosphorylates the α subunit of eukaryotic translation initiation factor 2 (EIF2S1/eIF-2α) in response to unfolded proteins and various cellular stresses.
  Related references:
  PMID:10026192, PMID:10677345, PMID:11907036
• Activation by misfolded proteins: It specifically recognizes and binds misfolded proteins in the ER, which triggers autophosphorylation and activation, initiating the integrated stress response (ISR).
  Related references:
  PMID:10677345, PMID:27917829, PMID:31023583
• Translation reprogramming: eIF2α phosphorylation attenuates global cap-dependent protein synthesis while selectively promoting translation of stress-responsive mRNAs, including the transcription factors ATF4 and QRICH1, thereby reprogramming gene expression.
  Related references:
  PMID:10026192, PMID:10677345, PMID:31023583
• Mitochondrial regulation: PERK-mediated UPR signaling increases mitochondrial oxidative phosphorylation by upregulating COX7A2L expression via ATF4. At mitochondria-ER contact sites, ATAD3A inhibits PERK to shield mitochondria from excessive UPR.
  Related references:
  PMID:31023583, PMID:39116259
• NRF2 activation: In addition to eIF2α, PERK phosphorylates NFE2L2/NRF2, causing its dissociation from KEAP1 and subsequent nuclear translocation, which activates antioxidant and detoxification genes (By similarity).
• Subcellular localization: Endoplasmic reticulum membrane – PERK is a transmembrane protein predominantly residing in the ER.
• Tissue distribution: Ubiquitously expressed, with particularly high levels in secretory tissues, consistent with its role in monitoring the ER folding environment.

Experimental Guidance and Technical Tips

• The immunogen corresponds to a region within amino acids 50–600 of human PERK. For IF/ICC, standard permeabilization and fixation conditions suitable for ER-resident proteins are recommended. Perform primary antibody titration to optimize signal-to-noise ratio.
• When using for ELISA, consider including a blocking peptide or recombinant PERK protein as a specificity control. Validate cross-reactivity with mouse samples using lysates from tissues with high secretory activity.
• Because PERK is an ER transmembrane kinase, consider using mild detergents during protein extraction to preserve conformational epitopes if the antibody is applied in other immunoassays beyond the validated applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic and research communities seamless access to validated IVD raw materials, including this anti-PERK polyclonal antibody for ER stress and UPR pathway studies. From optimized antibody pairs to bulk ancillary reagents, our supply chain covers every stage from concept to clinic. Reach out to our team today to discuss your specific sourcing requirements for rare targets and custom IVD development.