Perilipin-2 Rabbit Monoclonal Antibody for WB, ELISA - Q99541

Core Product Specifications and Parameters

Biological Background: Perilipin-2 Function and Localization

• Perilipin-2, encoded by the PLIN2 gene, is also known as Adipophilin or Adipose differentiation-related protein (ADRP).
• It functions as a structural component of lipid droplets, essential for their formation and maintenance.
• The protein localizes to the membrane of lipid droplets.
• Tissue expression includes milk lipid globules, highlighting its role in mammary gland lipid secretion.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation, as identified by proteomics.

Experimental Guidance and Technical Tips

• The antibody was raised against a synthetic peptide from human Perilipin-2; cross-reactivity with mouse and rat suggests epitope conservation across these species.
• For Western blot, a band at approximately 48 kDa is expected; consider using lipid-rich tissue lysates and mild lysis conditions to preserve antigen integrity.
• For ELISA, peptide-based immunoassays may be feasible; validate the antibody with recombinant protein or tissue extracts to confirm specificity.

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