Perilipin-2 Rabbit mAb - Q99541

Core Product Specifications and Parameters

Biological Background: Perilipin-2 Function and Localization

• Perilipin-2 (also known as Adipophilin, ADRP) is a lipid droplet-associated protein encoded by the PLIN2 gene.
• It serves as a structural component of lipid droplets, essential for their formation and maintenance.
• Located on the surface of lipid droplets (membrane-associated), it plays a key role in neutral lipid storage.
• Highly expressed in milk lipid globules, indicating a specialization in lipid secretion in mammary tissue.
• Post-translational modifications include acetylation, phosphorylation, and ubiquitin-like conjugation (Ubl), which may regulate its function.
• Identified in proteomics studies, confirming its presence as a reference proteome component.
• The protein has a molecular weight of approximately 48 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to amino acids 88-437, covering most of the protein, which may yield robust detection.
• For Western blot (WB), the predicted band is ~48 kDa; use an appropriate gel percentage and validate with a positive control.
• In immunohistochemistry (IHC-P), antigen retrieval with citrate buffer (pH 6.0) is commonly used for lipid droplet proteins.
• Immunofluorescence (IF/ICC) may require permeabilization with Triton X-100 or saponin to access intracellular lipid droplets.
• ELISA can be used for quantitative analysis; for sandwich ELISA, pair with a matched detection antibody.
• As a rabbit monoclonal antibody, ensure secondary antibody compatibility (e.g., anti-rabbit IgG HRP).

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, laboratories, and research institutes with one-stop access to IVD raw materials. For lipid metabolism and metabolic disorder research, we offer validated antibody pairs, optimized monoclonal antibodies like this Perilipin-2 antibody, and bulk ancillary reagents. Our sourcing expertise covers rare targets and custom solutions, supporting every stage from concept to clinic.