PerCP/Cyanine5.5 Rat anti-Mouse MHC Class II (I-A/I-E) Monoclonal Antibody for FC - P18468 / P06342 / Q3U060 / P14435

Core Product Specifications and Parameters

Biological Background: MHC Class II (I-A/I-E) Function and Localization

• The antibody targets mouse MHC class II I-A and I-E molecules, recognizing the I-A beta chain (encoded by H2-Eb1) as well as other alpha and beta chains (SWISS: P18468, P06342, Q3U060, P14435).
• It is a transmembrane glycoprotein located on the surface of professional antigen-presenting cells such as dendritic cells, macrophages, and B cells.
• MHC class II molecules play a central role in adaptive immunity by presenting peptides derived from extracellular antigens to CD4+ helper T cells.
• The protein contains a signal peptide, two extracellular domains (α1/β1 and α2/β2), a transmembrane helix, and a short cytoplasmic tail; it forms a heterodimer of alpha and beta chains.
• Keywords from UniProt include Adaptive immunity, Disulfide bond, Glycoprotein, MHC II, and Ubl conjugation, indicating post-translational modifications and functional classification.
• The antibody recognizes multiple haplotypes, making it broadly reactive across mouse strains expressing I-A and I-E alleles.

Experimental Guidance and Technical Tips

• The immunogen is recombinant mouse MHC Class II (I-A/I-E) protein; users may consider validating reactivity with their specific mouse strain or cell type of interest.
• For flow cytometry (FC), titrate the antibody to determine the optimal staining concentration for your experimental system.
• Ensure proper compensation when using PerCP/Cyanine5.5 in multicolor panels; avoid fixation/permeabilization steps that may affect conjugate brightness if not tested.
• Include appropriate isotype controls and viability dyes to gate live, single cells expressing MHC class II.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers, labs, and research institutes with one-stop access to IVD raw materials, technical services, and consulting. For MHC class II research and immunoassay development, we supply validated antibody pairs, optimized monoclonal/polyclonal antibodies, and bulk ancillary reagents. Contact us for rare target raw-material sourcing and customized support from concept to clinic.