PerCP/Cyanine5.5 Rabbit anti-Mouse CD68 Monoclonal Antibody for Flow Cytometry - P31996

Core Product Specifications and Parameters

Biological Background: CD68 (Macrosialin) Function and Localization

• CD68, also known as macrosialin, Lamp4, gp110, or Scard1, is a heavily glycosylated transmembrane protein belonging to the lysosomal-associated membrane protein (LAMP) family. Alternative splicing generates multiple isoforms.
• It plays a critical role in phagocytic activities of tissue macrophages, participating in intracellular lysosomal metabolism and extracellular interactions with pathogens or other cells.
• CD68 binds tissue- and organ-specific lectins or selectins, facilitating the homing of macrophage subsets to particular sites. Rapid recycling between endosomes, lysosomes, and the plasma membrane may support macrophage crawling on selectin-bearing substrates.
• Subcellular localization includes the endosome membrane, lysosome membrane, and cell membrane, consistent with its dynamic trafficking and function in antigen processing and presentation.
• It is predominantly expressed in tissue macrophages and, to a lesser extent, dendritic cells, serving as a classical marker for these myeloid lineages.
• Post-translational modifications include N-linked glycosylation and intramolecular disulfide bonds, which contribute to its structural stability and ligand-binding properties.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain of mouse CD68, potentially enabling recognition of surface and intracellular epitopes. For flow cytometry, consider using a live-cell surface staining protocol, with intracellular staining for total CD68 detection.
• As a directly conjugated antibody, it eliminates the need for secondary reagents, reducing assay time and potential cross-reactivity. Ensure appropriate isotype controls and Fc receptor blocking to minimize non-specific binding.
• Validate antibody performance in the relevant sample type (e.g., mouse primary macrophages, dendritic cells, or cell lines) and fixative/permeabilization conditions for intracellular applications.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw material procurement for diagnostic manufacturers and researchers, offering a comprehensive portfolio of antibodies, antigens, and ancillary reagents. Whether you need validated antibody pairs, bulk anti-CD68 conjugates, or rare-target sourcing for macrophage biology, CamelBio provides end-to-end support from concept to clinic.