PerCP/Cyanine5.5 Rabbit anti-Mouse CD4 Monoclonal Antibody for Flow Cytometry - P06332

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• The target is mouse CD4 (T-cell surface glycoprotein CD4), also known as L3T4 or Ly-4. Two alternatively spliced isoforms have been reported, with isoform 1 being the canonical sequence (UniProt P06332).
• CD4 is an integral membrane glycoprotein that functions as a coreceptor for MHC class II molecules in T-cells, recognizing peptides derived from extracellular proteins, and thereby playing an essential role in adaptive immunity.
• Upon engagement with MHC class II:peptide complexes on antigen-presenting cells, CD4 simultaneously interacts with the T-cell receptor (TCR) and recruits the Src kinase LCK to the TCR-CD3 complex, initiating signaling cascades that promote T-helper cell activation, cytokine production, and proliferation.
• Beyond TCR-dependent signaling, CD4 also contributes to TCR/LCK-independent functions in macrophages and NK cells, influencing differentiation, activation, cytokine expression, and cell migration.
• CD4 is critical for T-helper cell development in the thymus and is capable of triggering monocyte differentiation into functional mature macrophages.
• The protein is predominantly expressed on the cell membrane of T-helper cells, which are specialized in activating cytotoxic T-cells, regulating B cells, and activating phagocytes. It is also found in other immune cells such as macrophages, dendritic cells, and NK cells.
• Key post-translational modifications include N-linked glycosylation, palmitoylation, phosphorylation, and the presence of disulfide bonds, which are important for its structural stability and function.

Experimental Guidance and Technical Tips

• The immunogen used to generate this antibody spans amino acids 27–394 of mouse CD4 (NP_038516.1), which covers the entire extracellular domain. This design favors recognition of native surface-expressed CD4.
• This antibody is validated for flow cytometry (FC) and is supplied as a PerCP/Cyanine5.5 conjugate, enabling direct detection of mouse CD4 on live cells without the need for a secondary antibody.
• When setting up flow cytometry experiments, consider titrating the antibody to determine the optimal staining concentration for your specific cell type and instrument. Include appropriate isotype controls and viability dyes to ensure accurate gating.
• Since CD4 is expressed on T-helper cells and other immune populations, verify the staining pattern in the relevant mouse tissue or cell samples (e.g., splenocytes, thymocytes, PBMCs) and consider co-staining with CD3 and CD8 to discriminate T-cell subsets.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio is your one-stop sourcing partner for IVD raw materials, providing researchers and diagnostic developers with reliable access to validated antibody reagents. This anti-mouse CD4 monoclonal antibody addresses key needs in immunophenotyping and T-cell biology research. Beyond this product, CamelBio offers a comprehensive portfolio of monoclonal and polyclonal antibodies, bulk ancillary reagents, and custom sourcing support for rare targets, helping you streamline procurement from concept to clinic.