PerCP/Cyanine5.5 Rabbit anti-Mouse CD127/IL-7Rα Monoclonal Antibody for FC - P16872

Core Product Specifications and Parameters

Biological Background: CD127/IL-7Rα Function and Localization

• CD127, also known as interleukin-7 receptor subunit alpha (IL-7Rα), is a single-pass type I membrane protein that belongs to the type I cytokine receptor family.
• It functions as a specific receptor for interleukin-7 (IL-7), a cytokine critical for lymphocyte development and homeostasis.
• Additionally, CD127 acts as a receptor for thymic stromal lymphopoietin (TSLP), which plays a role in dendritic cell maturation and T cell responses.
• The receptor is predominantly expressed in lymphoid tissues including spleen, thymus, and fetal liver, consistent with its role in early lymphocyte development.
• CD127 is localized on the cell membrane, featuring an extracellular domain for ligand binding, a transmembrane helix, and a cytoplasmic tail involved in signaling.
• The protein undergoes post-translational modifications such as N-linked glycosylation and phosphorylation, and it contains disulfide bonds essential for its structural integrity.
• UniProt keywords include: 3D-structure, Disulfide bond, Glycoprotein, Membrane, Phosphoprotein, Receptor, Transmembrane, Transmembrane helix, and Ubl conjugation.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular domain (amino acids 21-239) of mouse CD127, which may favor detection of the native membrane-bound form in flow cytometry.
• For flow cytometry (FC), we recommend titrating the antibody to determine the optimal working concentration for your specific cell type and experimental conditions.
• Use appropriate positive (e.g., mouse thymocytes or splenocytes) and negative control cells to confirm specificity and minimal background staining.
• Protect the PerCP/Cyanine5.5 conjugate from prolonged exposure to light to avoid photobleaching, and handle according to standard fluorochrome-conjugated antibody storage guidelines.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio serves as a comprehensive sourcing partner for IVD raw materials, connecting diagnostic developers with high-quality reagents specific to immune targets like CD127/IL-7Rα. Our portfolio includes validated antibody pairs, optimized monoclonal antibodies suitable for flow cytometry, and ancillary reagents for immunoassay development. Whether you need bulk quantities or specialized rare-target reagents, CamelBio streamlines your path from concept to clinic.