PerCP/Cyanine5.5 Rabbit anti-Human/Monkey CD45 Monoclonal Antibody for Flow Cytometry - P08575

Core Product Specifications and Parameters

Biological Background: CD45 Function and Localization

• CD45, also known as leukocyte common antigen (L-CA) or T200, is a receptor-type tyrosine-protein phosphatase C encoded by the PTPRC gene. Multiple isoforms arise from alternative splicing, with molecular weights ranging from approximately 131 to 147 kDa.
• CD45 is essential for T-cell activation through the antigen receptor, acting as a positive regulator of coactivation upon binding to DPP4. It recruits and dephosphorylates SKAP1 and FYN, and modulates LYN activity (PubMed:35767951). Related references: PMID:35767951
• It functions as a protein tyrosine phosphatase, with the first PTPase domain exhibiting enzymatic activity and the second domain influencing substrate specificity.
• CD45 interacts with CLEC10A at the antigen-presenting cell–T cell contact, where CLEC10A on immature dendritic cells recognizes Tn antigen-carrying CD45 on effector T cells, modulating the T-cell activation threshold to limit autoreactivity.
• During cytomegalovirus infection, CD45 serves as a receptor for viral protein UL11, mediating binding to T-cells and leading to reduced tyrosine phosphorylation of signaling proteins and impaired T-cell proliferation.
• It localizes to the cell membrane, membrane rafts, and the immunological synapse, consistent with its role in signaling.
• CD45 is a glycoprotein and phosphoprotein, with key roles in host-virus interaction, and mutations are associated with SCID (severe combined immunodeficiency).

Experimental Guidance and Technical Tips

• The immunogen used is a recombinant fusion protein spanning amino acids 26-577 of human CD45 (P08575). This extracellular domain is commonly targeted for flow cytometry applications; consider this when designing your staining panel.
• For flow cytometry, titrate the antibody to determine optimal staining concentration. Include appropriate isotype controls and viability dyes. Since CD45 is expressed on all hematopoietic cells, careful gating strategies are advised to distinguish leukocyte subsets.
• The antibody cross-reacts with human and cynomolgus (Cynomolgus) CD45. When working with cynomolgus samples, validate the staining pattern in the relevant sample system to confirm reactivity and specificity.
• Multiple isoforms of CD45 exist (131-147 kDa), and the antibody may recognize various isoforms depending on the epitope location. If isoform-specific detection is required, confirm recognition by western blot or antigenic analysis.

CamelBio: Your One-Stop Sourcing Bridge

At CamelBio, we provide diagnostic manufacturers and research institutions with a seamless sourcing experience for IVD raw materials. This anti-CD45 antibody exemplifies our commitment to delivering high-quality monoclonal antibodies for immunophenotyping, supported by our extensive portfolio of validated antibody pairs, recombinant proteins, and ancillary reagents. Whether you need bulk quantities or assistance with rare target material sourcing, CamelBio is your trusted partner from concept to clinic.