PerCP/Cyanine5.5 Rabbit anti-Human/Monkey CD4 Monoclonal Antibody for FC - P01730

Core Product Specifications and Parameters

Biological Background: CD4 Function and Localization

• T-cell surface glycoprotein CD4, also known as T4 or Leu-3, is a member of the immunoglobulin superfamily and a hallmark marker of T-helper cells.
• Serves as a coreceptor for MHC class II molecules: it simultaneously binds the T-cell receptor (TCR) and MHC class II on antigen-presenting cells, recruiting the Src kinase LCK to initiate intracellular signaling pathways that lead to T-cell activation, lymphokine production, motility, and adhesion.
• Essential for thymic development of T-helper cells and triggers the differentiation of monocytes into functional mature macrophages.
• Acts as the primary receptor for human immunodeficiency virus-1 (HIV-1) by binding the viral envelope glycoprotein gp120, facilitating viral entry. Related references: PMID:12089508 PMID:16331979 PMID:2214026
• Also functions as a receptor for Human Herpesvirus 7 (HHV-7). Related references: PMID:7909607
• Down-regulated by HIV-1 Vpu protein to evade host immune response. Related references: PMID:17346169
• Highly expressed on the surface of T-helper cells, and also present on macrophages, dendritic cells, and NK cells.
• Subcellular localization: cell membrane; contains a single-pass transmembrane domain, disulfide bonds, glycosylation sites, and palmitoylation sites.

Experimental Guidance and Technical Tips

• The antibody is supplied as a ready-to-use PerCP/Cyanine5.5 conjugate, eliminating the need for secondary staining in flow cytometry (FC) assays.
• For FC, consider performing a titration experiment to determine the optimal working concentration in your sample system.
• Include appropriate isotype controls and fluorescence-minus-one (FMO) controls to ensure specificity.
• CD4 expression is most prominent on human T-helper cells; validate in peripheral blood mononuclear cells (PBMCs) or relevant cell lines. Cross-reactivity with Cynomolgus monkey allows for translational research.
• For fixation and permeabilization protocols, verify compatibility with PerCP/Cyanine5.5, as certain buffers may affect fluorophore stability.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio streamlines IVD raw-material procurement by offering a comprehensive portfolio of validated monoclonal antibodies, including this anti-CD4 clone for immune profiling and infectious disease research. As a single-source supplier, we pair premium antibody products with flexible sourcing solutions—from optimized antibody pairs for diagnostic kit development to bulk ancillary reagents—ensuring you have the reliable raw materials needed to move seamlessly from concept to clinic.