PerCP/Cyanine5.5 Rabbit anti-Human CD94 Monoclonal Antibody for Flow Cytometry - Q13241

Core Product Specifications and Parameters

Biological Background: CD94 Function and Localization

• CD94 (KLRD1) is a C-type lectin-like transmembrane receptor that forms disulfide-linked heterodimers with NKG2A (KLRC1) or NKG2C (KLRC2). These complexes recognize the non-classical MHC class I molecule HLA-E presenting self-peptides, enabling immune cells to monitor MHC class I expression. Related references: PMID:10023772, PMID:18064301, PMID:18083576, PMID:12387742, PMID:9430220

• The KLRD1-KLRC1 heterodimer serves as an inhibitory receptor. On NK cells, it recruits tyrosine phosphatases via ITIM motifs to dampen activation signals. On CD8+ T cells, it counteracts TCR signaling and promotes exhaustion in HLA-E-rich tumor microenvironments. Related references: PMID:30860984, PMID:9430220, PMID:9485206, PMID:12387742, PMID:18064301, PMID:30503213, PMID:12165520

• Conversely, the KLRD1-KLRC2 heterodimer acts as an activating receptor. It recognizes HLA-E presenting HLA-G-derived peptides and mediates adaptive NK cell responses to viral infections and maternal-fetal tolerance. Signaling occurs via the adaptor DAP12, leading to phosphorylation and cell activation. Related references: PMID:15940674, PMID:9655483, PMID:30134159, PMID:9754572, PMID:20952657, PMID:21825173

• CD94 is expressed on the plasma membrane of NK cells, memory/effector CD8+ αβ T cells, γδ T cells, and certain tumor-specific cytotoxic T lymphocytes. The expression pattern of inhibitory versus activating receptor complexes varies across lymphocyte subsets.

• During SARS-CoV-2 infection, the viral spike protein peptide can be presented by HLA-E and engage KLRD1-KLRC1, leading to functional exhaustion of cytotoxic NK cells and CD8+ T cells, thus impairing antiviral immune surveillance. Related references: PMID:32859121

• CD94 is a transmembrane glycoprotein with a single-pass type II orientation (signal-anchor), contains a C-type lectin-like domain, and features a disulfide bond. Alternative splicing produces multiple isoforms.

Experimental Guidance and Technical Tips

• The immunogen comprises amino acids 32-179 of human CD94, representing the extracellular C-type lectin-like domain. This domain design suggests the antibody may recognize native folded CD94 on the cell surface, making it suitable for flow cytometry applications.
• For flow cytometry, titrate the antibody to determine the optimal concentration for your sample type. Include appropriate isotype controls and consider using Fc receptor blocking to reduce non-specific binding.
• Since the antibody is directly conjugated to PerCP/Cyanine5.5, it is ready to use in multicolor panels. Verify compatibility with your instrument’s laser and filter configuration, and perform compensation adjustments as needed.
• The antibody is reported to cross-react with human CD94. Validate the assay in relevant human cell lines or primary NK/T cell populations to confirm reactivity in your specific experimental system.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio offers diagnostic manufacturers and research laboratories a streamlined sourcing solution for IVD raw materials. This anti-CD94 monoclonal antibody, along with complementary reagents like validated antibody pairs and ancillary buffers, supports immune checkpoint and NK cell research applications. For specialized requirements or bulk orders, our platform bridges your development needs with reliable, high-quality raw material supply.