PerCP/Cyanine5.5 Rabbit anti-Human CD62L/L-Selectin Monoclonal Antibody for FC - P14151

Core Product Specifications and Parameters

Biological Background: L-selectin Function and Localization

• L-selectin (SELL) is a calcium-dependent lectin that mediates cell adhesion by binding to glycoproteins on neighboring cells, playing a critical role in lymphocyte homing and leukocyte trafficking. Related references: PMID:12403782 PMID:28011641 PMID:28489325
• It promotes the initial tethering and rolling of leukocytes on endothelial cells, a key step in the leukocyte adhesion cascade. Related references: PMID:12403782 PMID:28011641
• Encoded by the SELL gene (alternative names: LNHR, LYAM1), the protein is also known as CD62L, LAM-1, Leu-8, and LECAM1.
• L-selectin is expressed on the surface of B cells and T lymphocytes, consistent with its role in immune surveillance.
• It is a transmembrane glycoprotein containing extracellular domains such as a C-type lectin domain, an EGF-like domain, and sushi (CCP) repeats.
• Post-translational modifications include glycosylation and disulfide bond formation, which are essential for its adhesive functions.
• The protein is localized to the cell membrane and acts as a homing receptor that directs lymphocytes to peripheral lymph nodes.
• Alternative initiation may produce isoforms with apparent molecular weights of approximately 42-43 kDa.

Experimental Guidance and Technical Tips

• The immunogen corresponds to the extracellular region (amino acids 39-332), ensuring recognition of the native surface antigen.
• As a directly conjugated antibody (PerCP/Cyanine5.5), it requires no secondary antibody and is optimized for flow cytometry.
• Titrate the antibody to determine the optimal staining concentration for your target cell type (e.g., human peripheral blood lymphocytes).
• Include appropriate isotype controls and fluorescence compensation controls in multicolor panels.
• Validate expression patterns in known positive (e.g., naïve T cells) and negative (e.g., some memory subsets) populations.

CamelBio: Your One-Stop Sourcing Bridge

CamelBio provides diagnostic manufacturers and research institutions with one-stop access to high-quality IVD raw materials. This PerCP/Cyanine5.5-conjugated CD62L antibody is a valuable tool for developing immunophenotyping assays in leukocyte adhesion and immune monitoring. Beyond this product, CamelBio can source validated antibody pairs, bulk ancillary reagents, and rare target raw materials to support your entire assay workflow.